Swartz:Protocols/Buffers

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To make own buffers for frequent cloning, these are the recipes:

Buffer P1 (resuspension):
50 mM Tris·Cl, pH 8.0
10 mM EDTA
100 µg/ml RNase A
Buffer P2 (Lysis)
200 mM NaOH
1% SDS (w/v)
Buffer N3 (Neutralization) – Proprietary but can be determined from Qiagen’s patent
4.2 M GuHCl,
0.9 M KAc, pH 4.8
PB (wash/binding buffer):
5.0 M GuHCl,
30% isopropanol
PE (wash buffer)
50mM Tris, pH 7.5 – Dilute 5X with pure ethanol
Buffer EB
10 mM Tris-Cl, pH 8.5
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