Swartz:Protocols

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==Protocols==
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=Protocols=
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==Lab Ordering==
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*[https://docs.google.com/spreadsheet/viewform?formkey=dGVtSVdMVnpiWWtGbU9FVjF6TUhPb2c6MQ#gid=0 USE THIS FORM]
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===Cloning===
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==Cloning==
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:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0 Swartz Lab Molecular Biology Handbook]
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:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook]
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library]
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library]
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:*[[Gibson_Assembly]]
'''DNA Design'''
'''DNA Design'''
:*[[Swartz:Protocols/Primer Design|Primer Design]]
:*[[Swartz:Protocols/Primer Design|Primer Design]]
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:*[[Swartz:Protocols/Gene Synthesis|Gene Synthesis]]
:*[[Swartz:Protocols/Gene Synthesis|Gene Synthesis]]
:*[[Swartz:Protocols/Restriction Cloning|Restriction Enzyme Digestion/Ligations]]
:*[[Swartz:Protocols/Restriction Cloning|Restriction Enzyme Digestion/Ligations]]
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:*[https://docs.google.com/document/d/1Z65LmK7fOUqnJ8_0A9xrUi-AUN2Vfru8VYR9WoLS_YM/edit Quikchange]
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:*[[Swartz:Protocols/Quikchange|Quikchange]]
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:*[https://docs.google.com/document/d/17lf6hG6u25vGYEW3y1CxoGyKHr-yJv9LbZeoYSGOw04/edit Gel Purification/PCR Cleanup]
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:*[[/Column_Regeneration|MaxiPrep Column Regeneration]]
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:*[[Genomic DNA Extraction|Genomic DNA Extraction]]
'''Expression Templates'''
'''Expression Templates'''
:*
:*
'''Plasmids'''
'''Plasmids'''
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:*[[Miniprep/Qiagen kit|Plasmid Miniprep]]
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:*[[Miniprep/Qiagen kit|OWW Plasmid Miniprep]] or [https://docs.google.com/document/d/1kX8-atjv8nRfLn1346UDbteZq_5_a2lVSzmtqU_XBYk/edit | Swartz Lab Version]
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:*[[Miniprep/Qiagen kit|Plasmid Miniprep]]
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:*[[/Maxiprep|Plasmid Maxiprep]]
'''Organisms'''
'''Organisms'''
:*[[Transforming chemically competent cells|E.coli Transformation]]
:*[[Transforming chemically competent cells|E.coli Transformation]]
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:*[[/Cloning| Other Cloning Protocols]]
:*[[/Cloning| Other Cloning Protocols]]
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:*[[Making_a_long_term_stock_of_bacteria| Creating an E. coli glycerol stock]]
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===Cell Free Protein Synthesis===
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==Cell Free Protein Synthesis==
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:*[[/Linear_DNA_Template|Preparing linear DNA template for CFPS]]
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:*[[/1L_extract_prep|1L Extract Prep]]
:*[[/Cell_Free|Cell Free Protein Synthesis]]
:*[[/Cell_Free|Cell Free Protein Synthesis]]
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:*[https://docs.google.com/document/d/1vgVufOvnKvn84drFBmOtEqhZQEKNFzKqw82ItypqvHI/ How to do an Autoradiogram]
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:*[[/Autoradiogram|How to do an Autoradiogram]]
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:*[[/Western|Western Blotting]]
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:*[[/HD_Extract_Prep|High Density Fermentation]]
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:*[[/otDNA|Orthogonal tDNA template prep]]
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:*[[/Protein_gels|Running a protein gel]]
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**[[S30_buffer]]
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===Assays===
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==Protein Purification==
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:*[[/Purification_of_His-tagged_proteins|His-Tag Purification]]
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:*[[/Ribosome_purification|Ribosome purification]]
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==Assays==
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:*[[Nanodrop]]
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:*[[DNA_Quantification | Using the spec for DNA Quantification]]
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:*[[/Mass_spec| Sample prep for MS]]
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:*[[/Ribosome_binding| Spin assay for ribsome binding]]
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:*[[/HPLC|HPLC]]
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:*[[Free_Sulfhydryl_Determination| Ellman's Reagent Test]]
:*[[Swartz:Protocols/Cytochrome C|Cytochrome C ]]
:*[[Swartz:Protocols/Cytochrome C|Cytochrome C ]]
:*[[Swartz:Protocols/Methyl Viologen|Methyl Viologen]]
:*[[Swartz:Protocols/Methyl Viologen|Methyl Viologen]]
:*[[Swartz:Protocols/H2 Production (GC)|H2 Production (GC)]]
:*[[Swartz:Protocols/H2 Production (GC)|H2 Production (GC)]]
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===Miscellaneous===
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==Miscellaneous==
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:*[https://docs.google.com/document/d/1nlZCY_zG3eyBWw3DReVQHMm5oV88eX7cJpdavRdB2cw/edit Anaerobic Protein Production]
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:*[https://docs.google.com/document/d/1nlZCY_zG3eyBWw3DReVQHMm5oV88eX7cJpdavRdB2cw/edit Anaerobic Protein Production in E.coli]
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:*[[/A20_cells|Cell culture for A20 B-cells]]
==Solutions==
==Solutions==
:*[[/Buffers|Buffer Recipes]]
:*[[/Buffers|Buffer Recipes]]
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[[Category:Swartz_Protocols]]

Current revision

Swartz
Research Papers People Contact


Getting settled in the Swartz Lab


Contents

Protocols

Lab Ordering

Cloning

DNA Design

Gene Synthesis

Expression Templates

Plasmids

Organisms

Cell Free Protein Synthesis

Protein Purification

Assays

Miscellaneous

Solutions

Personal tools