Swartz:Protocols

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==Protocols==
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=Protocols=
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===Cloning===
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==Cloning==
:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook]
:*[https://docs.google.com/document/d/1KHeoV1WNDpWqD-90k0X5MtVM1naTK5_81uctSkKF5z0/edit Swartz Lab Molecular Biology Handbook]
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library]
:*[https://docs.google.com/spreadsheet/ccc?key=0AuGtz3V56Y9xdGQyaEUtM1p1TTh1dmQtZHhLUWVpclE&pli=1#gid=5 Restriction Enzyme Library]
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:*[[/Cloning| Other Cloning Protocols]]
:*[[/Cloning| Other Cloning Protocols]]
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===Cell Free Protein Synthesis===
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==Cell Free Protein Synthesis==
:*[[/Linear_DNA_Template|Preparing linear DNA template for CFPS]]
:*[[/Linear_DNA_Template|Preparing linear DNA template for CFPS]]
:*[[/1L_extract_prep|1L Extract Prep]]
:*[[/1L_extract_prep|1L Extract Prep]]
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:*[[/Western|Western Blotting]]
:*[[/Western|Western Blotting]]
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===Protein Purification===
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==Protein Purification==
:*[[/Purification_of_His-tagged_proteins|His-Tag Purification]]
:*[[/Purification_of_His-tagged_proteins|His-Tag Purification]]
:*[[/Ribosome_purification|Ribosome purification]]
:*[[/Ribosome_purification|Ribosome purification]]
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===Assays===
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==Assays==
:*[[Nanodrop]]
:*[[Nanodrop]]
:*[[/Mass_spec| Sample prep for MS]]
:*[[/Mass_spec| Sample prep for MS]]
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:*[[Swartz:Protocols/H2 Production (GC)|H2 Production (GC)]]
:*[[Swartz:Protocols/H2 Production (GC)|H2 Production (GC)]]
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===Miscellaneous===
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==Miscellaneous==
:*[https://docs.google.com/document/d/1nlZCY_zG3eyBWw3DReVQHMm5oV88eX7cJpdavRdB2cw/edit Anaerobic Protein Production]
:*[https://docs.google.com/document/d/1nlZCY_zG3eyBWw3DReVQHMm5oV88eX7cJpdavRdB2cw/edit Anaerobic Protein Production]
:*[[/A20_cells|Cell culture for A20 B-cells]]
:*[[/A20_cells|Cell culture for A20 B-cells]]

Revision as of 02:30, 15 May 2012

Swartz
Research Papers People Contact


Getting settled in the Swartz Lab


Contents

Protocols

Cloning

DNA Design

Gene Synthesis

Expression Templates

Plasmids

Organisms

Cell Free Protein Synthesis

Protein Purification

Assays

Miscellaneous

Solutions

Personal tools