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Streptomycin - Revision history
2013-05-20T16:56:36Z
Revision history for this page on the wiki
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Reshma P. Shetty at 17:48, 26 July 2007
2007-07-26T17:48:37Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:48, 26 July 2007</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.</div></td></tr>
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Reshma P. Shetty
http://openwetware.org/index.php?title=Streptomycin&diff=16370&oldid=prev
Tk: /* Mode of Action */
2006-01-07T16:20:37Z
<p><span class="autocomment">Mode of Action</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 16:20, 7 January 2006</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Inhibits protein synthesis; binds to 30S ribosomal subunit.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Inhibits protein synthesis; binds to 30S ribosomal subunit.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The rpsL50 gene mutation conveys streptomycin resistance. Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity. This allows counter-selection against the presence of the wt rpsL gene. Mutations of wild type genes to convey resistance are common.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Mechanism of Resistance==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Mechanism of Resistance==</div></td></tr>
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Tk
http://openwetware.org/index.php?title=Streptomycin&diff=11571&oldid=prev
Reshma P. Shetty: /* Usage Notes */
2005-05-24T15:02:29Z
<p><span class="autocomment">Usage Notes</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:02, 24 May 2005</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Usage Notes==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Usage Notes==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#100 mg/mL stock solution in H<sub>2</sub>O. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#100 mg/mL stock solution in H<sub>2</sub>O. <ins class="diffchange diffchange-inline"><br> It is barely soluble in ethanol so you must dissolve it in H<sub>2</sub>O.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Filter-sterilize with 0.22 &mu;m filter. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Filter-sterilize with 0.22 &mu;m filter. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Add 1 mL per liter of culture.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Add 1 mL per liter of culture.</div></td></tr>
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Reshma P. Shetty
http://openwetware.org/index.php?title=Streptomycin&diff=583&oldid=prev
Reshma P. Shetty at 20:28, 23 May 2005
2005-05-23T20:28:12Z
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<p><b>New page</b></p><div>==Mode of Action==<br />
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Inhibits protein synthesis; binds to 30S ribosomal subunit.<br />
<br />
==Mechanism of Resistance==<br />
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==Usage Notes==<br />
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#100 mg/mL stock solution in H<sub>2</sub>O. <br />
#Filter-sterilize with 0.22 &mu;m filter. <br />
#Add 1 mL per liter of culture.<br />
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==References==<br />
<br />
J. H. Miller. ''A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria.'' Cold Spring Harbor Laboratory Press, 1992.<br />
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J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001.</div>
Reshma P. Shetty