Streptomycin

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Usage Notes)
(Mode of Action)
Line 2: Line 2:
Inhibits protein synthesis; binds to 30S ribosomal subunit.
Inhibits protein synthesis; binds to 30S ribosomal subunit.
 +
 +
The rpsL50 gene mutation conveys streptomycin resistance.  Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity.  This allows counter-selection against the presence of the wt rpsL gene.  Mutations of wild type genes to convey resistance are common.
==Mechanism of Resistance==
==Mechanism of Resistance==

Revision as of 11:20, 7 January 2006

Contents

Mode of Action

Inhibits protein synthesis; binds to 30S ribosomal subunit.

The rpsL50 gene mutation conveys streptomycin resistance. Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity. This allows counter-selection against the presence of the wt rpsL gene. Mutations of wild type genes to convey resistance are common.

Mechanism of Resistance

Usage Notes

  1. 100 mg/mL stock solution in H2O.
    It is barely soluble in ethanol so you must dissolve it in H2O.
  2. Filter-sterilize with 0.22 μm filter.
  3. Add 1 mL per liter of culture.

References

J. H. Miller. A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. Cold Spring Harbor Laboratory Press, 1992.

J. Sambrook and D. W. Russell. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 3rd edition, 2001.

Personal tools