Streptomycin: Difference between revisions
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Inhibits protein synthesis; binds to 30S ribosomal subunit. | Inhibits protein synthesis; binds to 30S ribosomal subunit. | ||
The rpsL50 gene mutation conveys streptomycin resistance. Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity. This allows counter-selection against the presence of the wt rpsL gene. Mutations of wild type genes to convey resistance are common. | |||
==Mechanism of Resistance== | ==Mechanism of Resistance== | ||
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==Usage Notes== | ==Usage Notes== | ||
#100 mg/mL stock solution in H<sub>2</sub>O. | #100 mg/mL stock solution in H<sub>2</sub>O. <br> It is barely soluble in ethanol so you must dissolve it in H<sub>2</sub>O. | ||
#Filter-sterilize with 0.22 μm filter. | #Filter-sterilize with 0.22 μm filter. | ||
#Add 1 mL per liter of culture. | #Add 1 mL per liter of culture. | ||
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J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001. | J. Sambrook and D. W. Russell. ''Molecular Cloning: A Laboratory Manual.'' Cold Spring Harbor Laboratory Press, 3rd edition, 2001. | ||
[[Category:Material]] [[Category:Antibiotic]] | |||
Latest revision as of 10:48, 26 July 2007
Mode of Action
Inhibits protein synthesis; binds to 30S ribosomal subunit.
The rpsL50 gene mutation conveys streptomycin resistance. Resistance is recessive, so the presence of a wild type rpsL gene will convey sensitivity. This allows counter-selection against the presence of the wt rpsL gene. Mutations of wild type genes to convey resistance are common.
Mechanism of Resistance
Usage Notes
- 100 mg/mL stock solution in H2O.
It is barely soluble in ethanol so you must dissolve it in H2O. - Filter-sterilize with 0.22 μm filter.
- Add 1 mL per liter of culture.
References
J. H. Miller. A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. Cold Spring Harbor Laboratory Press, 1992.
J. Sambrook and D. W. Russell. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, 3rd edition, 2001.
