Streptomyces:Other Bits/Antibiotic and Resistance Marker Information - Revision history

Streptomyces at 14:40, 31 August 2007

Streptomyces — Fri, 31 Aug 2007 14:40:21 GMT

←Older revision		Revision as of 14:40, 31 August 2007
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	[[Category:Escherichia coli]]		[[Category:Escherichia coli]]
	[[Category:Streptomyces]]		[[Category:Streptomyces]]
		+	[[Category:Antibiotic]]
	<!--CONTENT START-->		<!--CONTENT START-->

Streptomyces at 13:03, 14 August 2007

Streptomyces — Tue, 14 Aug 2007 13:03:20 GMT

←Older revision		Revision as of 13:03, 14 August 2007
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	<!--INSERT THE TEMPLATE HEADER-->		<!--INSERT THE TEMPLATE HEADER-->
	{{Streptomyces}}		{{Streptomyces}}
-	~~<br>~~
	{\|cellspacing="5" cellpadding="10" style="background:#000; width: 800px;"		{\|cellspacing="5" cellpadding="10" style="background:#000; width: 800px;"
	\|-valign="top"		\|-valign="top"

Streptomyces at 11:41, 10 August 2007

Streptomyces — Fri, 10 Aug 2007 11:41:42 GMT

←Older revision		Revision as of 11:41, 10 August 2007
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	\| DH5α \|\| BT340 (FLP Recombinase) \|\| None \|\| ''cat'' / ''bla<sub>carb</sub>'' \|\| Initial Replication @ 30°C, <br/>Induction of FLP Recombinase & loss of plasmid @ 42°C, <br/>Post recombination growth @37°C		\| DH5α \|\| BT340 (FLP Recombinase) \|\| None \|\| ''cat'' / ''bla<sub>carb</sub>'' \|\| Initial Replication @ 30°C, <br/>Induction of FLP Recombinase & loss of plasmid @ 42°C, <br/>Post recombination growth @37°C
	\|- style="text-align:center;"		\|- style="text-align:center;"
-	\| ET12567 [dam-13::Tn9] \|\| pUZ8002 \|\| ''cat'' \|\| ''neo'' \|\|	+	\| ET12567 <br/>[dam-13::Tn9] \|\| pUZ8002 \|\| ''cat'' \|\| ''neo'' \|\|
	\|- style="text-align:center;"		\|- style="text-align:center;"
	\| \|\| SuperCos 1 \|\| \|\| ''neo'' / ''bla<sub>carb</sub>'' \|\|		\| \|\| SuperCos 1 \|\| \|\| ''neo'' / ''bla<sub>carb</sub>'' \|\|

Streptomyces at 11:39, 10 August 2007

Streptomyces — Fri, 10 Aug 2007 11:39:51 GMT

←Older revision		Revision as of 11:39, 10 August 2007
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	\|- style="text-align:center;" valign="top"		\|- style="text-align:center;" valign="top"
	! style="width:175px;" \| Name \|\| style="width:150px;" \| Stock Concentration (mg/ml) \|\| style="width:150px;"\| Final Concentration (μg/ml) \|\| style="width:100px;"\| Usage in 10mL (μL) \|\| Dissolve in		! style="width:175px;" \| Name \|\| style="width:150px;" \| Stock Concentration (mg/ml) \|\| style="width:150px;"\| Final Concentration (μg/ml) \|\| style="width:100px;"\| Usage in 10mL (μL) \|\| Dissolve in
-	\|- style="text-align:center;"	+	\|- style="text-align:center; border-top: 2px solid black;"
	\| Ampicillin \|\| 100 \|\| 100 \|\| 10 \|\| sdH<sub>2</sub>0		\| Ampicillin \|\| 100 \|\| 100 \|\| 10 \|\| sdH<sub>2</sub>0
	\|- style="text-align:center;"		\|- style="text-align:center;"
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	\| Viomycin \|\| 30 \|\| 30 \|\| 10 \|\| sdH<sub>2</sub>0		\| Viomycin \|\| 30 \|\| 30 \|\| 10 \|\| sdH<sub>2</sub>0
	\|}		\|}
-		+	<br/>
		+	<br/>
		+	<br/>

	<big>'''E.coli Strain & Plasmid Resistance Markers'''</big>		<big>'''E.coli Strain & Plasmid Resistance Markers'''</big>
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	\|- style="text-align:center;" valign="top"		\|- style="text-align:center;" valign="top"
	! style="width:130px;" \| E.coli Strain \|\| style="width:120px;" \| Plasmid / Cosmid \|\| style="width:120px;"\| E.coli Resistance Marker \|\| style="width:100px;"\| Plasmid Resistance Marker \|\| Temperature Sensitive Replicon <br/>(t<sup>s</sup>)		! style="width:130px;" \| E.coli Strain \|\| style="width:120px;" \| Plasmid / Cosmid \|\| style="width:120px;"\| E.coli Resistance Marker \|\| style="width:100px;"\| Plasmid Resistance Marker \|\| Temperature Sensitive Replicon <br/>(t<sup>s</sup>)
-	\|- style="text-align:center;"	+	\|- style="text-align:center; border-top: 2px solid black;"
	\| BW25113 \|\| pIJ790 (λRED) \|\| None \|\| ''cat'' \|\| Replication @ 30°C, <br/>Loss of λRED @ 37°C		\| BW25113 \|\| pIJ790 (λRED) \|\| None \|\| ''cat'' \|\| Replication @ 30°C, <br/>Loss of λRED @ 37°C
	\|- style="text-align:center;"		\|- style="text-align:center;"
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	\| \|\| pIJ773 \|\| \|\| ''acc(3)IV'' \|\|		\| \|\| pIJ773 \|\| \|\| ''acc(3)IV'' \|\|
	\|}		\|}
-		+	<br/>
		+	<br/>
		+	<br/>

	<big>'''Antibiotic Genes & Information'''</big>		<big>'''Antibiotic Genes & Information'''</big>
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	\|- style="text-align:center;" valign="top"		\|- style="text-align:center;" valign="top"
	! style="width:225px;" \| Antibiotic Name \|\| style="width:125px;" \| Gene Name \|\| style="width:125px;"\| Type \|\| Common Form		! style="width:225px;" \| Antibiotic Name \|\| style="width:125px;" \| Gene Name \|\| style="width:125px;"\| Type \|\| Common Form
-	\|- style="text-align:center;"	+	\|- style="text-align:center; border-top: 2px solid black;"
	\| Ampicillin \|\| ''bla'' \|\| Bactericidal \|\| Sodium		\| Ampicillin \|\| ''bla'' \|\| Bactericidal \|\| Sodium
	\|- style="text-align:center;"		\|- style="text-align:center;"

Streptomyces: Streptomyces:Media and Solutions/Antibiotic and Resistance Marker Information moved to Streptomyces:Other Bits/Antibiotic and Resistance Marker Information: Re-categorised

Streptomyces — Fri, 10 Aug 2007 11:15:42 GMT

Streptomyces:Media and Solutions/Antibiotic and Resistance Marker Information moved to Streptomyces:Other Bits/Antibiotic and Resistance Marker Information: Re-categorised

←Older revision

Revision as of 11:15, 10 August 2007

Streptomyces at 11:14, 10 August 2007

Streptomyces — Fri, 10 Aug 2007 11:14:34 GMT

←Older revision		Revision as of 11:14, 10 August 2007
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-	==~~Media and Solutions~~ - Antibiotic & Resistance Marker Information==	+	==Other Bits - Antibiotic & Resistance Marker Information==
	<!--INSERT THE TEMPLATE HEADER-->		<!--INSERT THE TEMPLATE HEADER-->
	{{Streptomyces}}		{{Streptomyces}}

Streptomyces at 11:12, 10 August 2007

Streptomyces — Fri, 10 Aug 2007 11:12:29 GMT

←Older revision		Revision as of 11:12, 10 August 2007
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	\|style="background:#ffffff"\|		\|style="background:#ffffff"\|
	<!--THESE CATEGORY LINES ADD THE PROTOCOL TO THE OWW PROTOCOLS PAGE-->		<!--THESE CATEGORY LINES ADD THE PROTOCOL TO THE OWW PROTOCOLS PAGE-->
-	[[Category:~~Materials~~]]	+	[[Category:Material]]
	[[Category:Escherichia coli]]		[[Category:Escherichia coli]]
	[[Category:Streptomyces]]		[[Category:Streptomyces]]

Streptomyces at 11:11, 10 August 2007

Streptomyces — Fri, 10 Aug 2007 11:11:53 GMT

←Older revision		Revision as of 11:11, 10 August 2007
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	\|-valign="top"		\|-valign="top"
	\|style="background:#ffffff"\|		\|style="background:#ffffff"\|
		+	<!--THESE CATEGORY LINES ADD THE PROTOCOL TO THE OWW PROTOCOLS PAGE-->
		+	[[Category:Materials]]
		+	[[Category:Escherichia coli]]
		+	[[Category:Streptomyces]]
	<!--CONTENT START-->		<!--CONTENT START-->

Streptomyces at 21:41, 29 July 2007

Streptomyces — Sun, 29 Jul 2007 21:41:57 GMT

←Older revision		Revision as of 21:41, 29 July 2007
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	<!--CONTENT START-->		<!--CONTENT START-->

-	~~===~~Antibiotic Concentrations and Usage~~===~~	+	<big>'''Antibiotic Concentrations and Usage'''</big>

	{\|cellspacing="0" cellpadding="5" rules="rows" frame="hsides" style="width: 770px;"		{\|cellspacing="0" cellpadding="5" rules="rows" frame="hsides" style="width: 770px;"
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-	~~===~~E.coli Strain & Plasmid Resistance Markers~~===~~	+	<big>'''E.coli Strain & Plasmid Resistance Markers'''</big>

	{\|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides"		{\|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides"
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-	~~===~~Antibiotic Genes & Information~~===~~	+	<big>'''Antibiotic Genes & Information'''</big>

	{\|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides"		{\|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides"

Streptomyces at 12:39, 27 July 2007

Streptomyces — Fri, 27 Jul 2007 12:39:41 GMT

←Older revision		Revision as of 12:39, 27 July 2007
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	{\|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides"		{\|cellspacing="0" cellpadding="5" style="width: 770px;" rules="rows" frame="hsides"
	\|- style="text-align:center;" valign="top"		\|- style="text-align:center;" valign="top"
-	! style="width:130px;" \| E.coli Strain \|\| style="width:120px;" \| Plasmid / Cosmid \|\| style="width:120px;"\| E.coli Resistance Marker \|\| style="width:100px;"\| Plasmid Resistance Marker \|\| Temperature Sensitive Replicon (ts)	+	! style="width:130px;" \| E.coli Strain \|\| style="width:120px;" \| Plasmid / Cosmid \|\| style="width:120px;"\| E.coli Resistance Marker \|\| style="width:100px;"\| Plasmid Resistance Marker \|\| Temperature Sensitive Replicon <br/>(t<sup>s</sup>)
	\|- style="text-align:center;"		\|- style="text-align:center;"
	\| BW25113 \|\| pIJ790 (λRED) \|\| None \|\| ''cat'' \|\| Replication @ 30°C, <br/>Loss of λRED @ 37°C		\| BW25113 \|\| pIJ790 (λRED) \|\| None \|\| ''cat'' \|\| Replication @ 30°C, <br/>Loss of λRED @ 37°C