Streptomyces:Other Bits/Antibiotic and Resistance Marker Information

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| DH5α || BT340 (FLP Recombinase) || None || ''cat'' / ''bla<sub>carb</sub>'' || Initial Replication @ 30&#176;C, <br/>Induction of FLP Recombinase & loss of plasmid @ 42&#176;C, <br/>Post recombination growth @37&#176;C
| DH5α || BT340 (FLP Recombinase) || None || ''cat'' / ''bla<sub>carb</sub>'' || Initial Replication @ 30&#176;C, <br/>Induction of FLP Recombinase & loss of plasmid @ 42&#176;C, <br/>Post recombination growth @37&#176;C
|- style="text-align:center;"
|- style="text-align:center;"
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| ET12567 [dam-13::Tn9] || pUZ8002 || ''cat'' || ''neo'' ||
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| ET12567 <br/>[dam-13::Tn9] || pUZ8002 || ''cat'' || ''neo'' ||
|- style="text-align:center;"
|- style="text-align:center;"
| || SuperCos 1 || || ''neo'' / ''bla<sub>carb</sub>'' ||  
| || SuperCos 1 || || ''neo'' / ''bla<sub>carb</sub>'' ||  

Current revision

Other Bits - Antibiotic & Resistance Marker Information

Streptomyces @ UEA

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Antibiotic Concentrations and Usage

Name Stock Concentration (mg/ml) Final Concentration (μg/ml) Usage in 10mL (μL) Dissolve in
Ampicillin 100 100 10 sdH20
Apramycin 100 50 5 sdH20
Chloramphenicol 25 25 10 50%-100% EtOH
Hygromycin 50 25 5 sdH20
Kanamycin 100 50 5 sdH20
Nalidixic Acid 25 25 10 0.2M NaOH
Thiostrepton(e) 50 50 10 100% DMSO or 1:1 DMSO:EtOH
Viomycin 30 30 10 sdH20




E.coli Strain & Plasmid Resistance Markers

E.coli Strain Plasmid / Cosmid E.coli Resistance Marker Plasmid Resistance Marker Temperature Sensitive Replicon
(ts)
BW25113 pIJ790 (λRED) None cat Replication @ 30°C,
Loss of λRED @ 37°C
DH5α None N/A
DH5α BT340 (FLP Recombinase) None cat / blacarb Initial Replication @ 30°C,
Induction of FLP Recombinase & loss of plasmid @ 42°C,
Post recombination growth @37°C
ET12567
[dam-13::Tn9] 		pUZ8002 cat neo
SuperCos 1 neo / blacarb
pIJ773 acc(3)IV




Antibiotic Genes & Information

Antibiotic Name Gene Name Type Common Form
Ampicillin bla Bactericidal Sodium
Apramycin aac(3)IV Aminoglycoside Sulphate
Carbenicillin blacarb Bactericidal DiSodium
Chloramphenicol cat Bacteriostatic
Erythromycin ermE Macrolides
Hygromycin B hph Aminoglycoside
Kanamycin / Neomycin neo Aminoglycoside MonoSulphate
Nalidixic Acid Synthetic Bacteriostatic
Spectinomycin / Streptomycin aadA Aminocyclitol DiHydrochloride Pentahydrate
Tetracyclin Tet Aminoglycoside Hydrochloride
Thiostrepton tsr Aminoglycoside
Viomycin vph Aminoglycoside? Sulphate
Vancomycin B vanB Glycopeptide Hydrochloride



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