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Revision as of 07:15, 10 August 2007
Other Bits - Antibiotic & Resistance Marker Information
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Antibiotic Concentrations and Usage
| Name | Stock Concentration (mg/ml) | Final Concentration (μg/ml) | Usage in 10mL (μL) | Dissolve in
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| Ampicillin | 100 | 100 | 10 | sdH20
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| Apramycin | 100 | 50 | 5 | sdH20
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| Chloramphenicol | 25 | 25 | 10 | 50%-100% EtOH
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| Hygromycin | 50 | 25 | 5 | sdH20
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| Kanamycin | 100 | 50 | 5 | sdH20
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| Nalidixic Acid | 25 | 25 | 10 | 0.2M NaOH
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| Thiostrepton(e) | 50 | 50 | 10 | 100% DMSO or 1:1 DMSO:EtOH
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| Viomycin | 30 | 30 | 10 | sdH20
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E.coli Strain & Plasmid Resistance Markers
| E.coli Strain | Plasmid / Cosmid | E.coli Resistance Marker | Plasmid Resistance Marker | Temperature Sensitive Replicon
(ts)
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| BW25113 | pIJ790 (λRED) | None | cat | Replication @ 30°C,
Loss of λRED @ 37°C
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| DH5α | None | N/A | |
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| DH5α | BT340 (FLP Recombinase) | None | cat / blacarb | Initial Replication @ 30°C,
Induction of FLP Recombinase & loss of plasmid @ 42°C,
Post recombination growth @37°C
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| ET12567 [dam-13::Tn9] | pUZ8002 | cat | neo |
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| | SuperCos 1 | | neo / blacarb |
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| | pIJ773 | | acc(3)IV |
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Antibiotic Genes & Information
| Antibiotic Name | Gene Name | Type | Common Form
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| Ampicillin | bla | Bactericidal | Sodium
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| Apramycin | aac(3)IV | Aminoglycoside | Sulphate
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| Carbenicillin | blacarb | Bactericidal | DiSodium
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| Chloramphenicol | cat | Bacteriostatic |
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| Erythromycin | ermE | Macrolides |
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| Hygromycin B | hph | Aminoglycoside |
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| Kanamycin / Neomycin | neo | Aminoglycoside | MonoSulphate
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| Nalidixic Acid | Synthetic | Bacteriostatic |
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| Spectinomycin / Streptomycin | aadA | Aminocyclitol | DiHydrochloride Pentahydrate
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| Tetracyclin | Tet | Aminoglycoside | Hydrochloride
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| Thiostrepton | tsr | Aminoglycoside |
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| Viomycin | vph | Aminoglycoside? | Sulphate
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| Vancomycin B | vanB | Glycopeptide | Hydrochloride
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