Streptomyces:Other Bits/Antibiotic and Resistance Marker Information

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Revision as of 07:12, 10 August 2007

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Antibiotic Concentrations and Usage

Name	Stock Concentration (mg/ml)	Final Concentration (μg/ml)	Usage in 10mL (μL)	Dissolve in
Ampicillin	100	100	10	sdH₂0
Apramycin	100	50	5	sdH₂0
Chloramphenicol	25	25	10	50%-100% EtOH
Hygromycin	50	25	5	sdH₂0
Kanamycin	100	50	5	sdH₂0
Nalidixic Acid	25	25	10	0.2M NaOH
Thiostrepton(e)	50	50	10	100% DMSO or 1:1 DMSO:EtOH
Viomycin	30	30	10	sdH₂0

E.coli Strain & Plasmid Resistance Markers

E.coli Strain	Plasmid / Cosmid	E.coli Resistance Marker	Plasmid Resistance Marker	Temperature Sensitive Replicon (t^s)
BW25113	pIJ790 (λRED)	None	cat	Replication @ 30°C, Loss of λRED @ 37°C
DH5α	None	N/A
DH5α	BT340 (FLP Recombinase)	None	cat / bla_carb	Initial Replication @ 30°C, Induction of FLP Recombinase & loss of plasmid @ 42°C, Post recombination growth @37°C
ET12567 [dam-13::Tn9]	pUZ8002	cat	neo
	SuperCos 1		neo / bla_carb
	pIJ773		acc(3)IV

Antibiotic Genes & Information

Antibiotic Name	Gene Name	Type	Common Form
Ampicillin	bla	Bactericidal	Sodium
Apramycin	aac(3)IV	Aminoglycoside	Sulphate
Carbenicillin	bla_carb	Bactericidal	DiSodium
Chloramphenicol	cat	Bacteriostatic
Erythromycin	ermE	Macrolides
Hygromycin B	hph	Aminoglycoside
Kanamycin / Neomycin	neo	Aminoglycoside	MonoSulphate
Nalidixic Acid	Synthetic	Bacteriostatic
Spectinomycin / Streptomycin	aadA	Aminocyclitol	DiHydrochloride Pentahydrate
Tetracyclin	Tet	Aminoglycoside	Hydrochloride
Thiostrepton	tsr	Aminoglycoside
Viomycin	vph	Aminoglycoside?	Sulphate
Vancomycin B	vanB	Glycopeptide	Hydrochloride

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