Streptomyces:Other Bits/Antibiotic and Resistance Marker Information: Difference between revisions
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===Antibiotic Concentrations and Usage=== | ===Antibiotic Concentrations and Usage=== | ||
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! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ||
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! style="width:175px;" | E.coli Strain || style="width:150px;" | Plasmid / Cosmid || style="width:150px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts) | ! style="width:175px;" | E.coli Strain || style="width:150px;" | Plasmid / Cosmid || style="width:150px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts) | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| BW25113 || pIJ790 (λRED) || None || cat || Replication @ 30C, Loss of λRED @ 37C | | BW25113 || pIJ790 (λRED) || None || cat || Replication @ 30C, Loss of λRED @ 37C | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| DH5α || None || N/A || || | | DH5α || None || N/A || || | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| DH5α || BT340 (FLP Recombinase) || None || cat / blacarb || Initial Replication @ 30C, Induction of FLP Recombinase & loss of plasmid @ 42C, Post recombination growth @37C | | DH5α || BT340 (FLP Recombinase) || None || cat / blacarb || Initial Replication @ 30C, Induction of FLP Recombinase & loss of plasmid @ 42C, Post recombination growth @37C | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| ET12567 [dam-13::Tn9] || pUZ8002 || cat || neo || | | ET12567 [dam-13::Tn9] || pUZ8002 || cat || neo || | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| || SuperCos 1 || || neo / blacarb || | | || SuperCos 1 || || neo / blacarb || | ||
|- style="text-align:center | |- style="text-align:center;" | ||
| || pIJ773 || || acc(3)IV || | | || pIJ773 || || acc(3)IV || | ||
|} | |} |
Revision as of 03:37, 27 July 2007
Media and Solutions - Antibiotic & Resistance Marker Information
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Antibiotic Concentrations and Usage
E.coli Strain & Plasmid Resistance Markers
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