Stevej:Cell counting/hemocytometer: Difference between revisions
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#Mean cell count/4 * Dilution = Cells x 10<sup>4</sup>/ml. | #Mean cell count/4 * Dilution = Cells x 10<sup>4</sup>/ml. | ||
#Above example, Mean cell count/4 * 2 = Cells x 10<sup>4</sup>/ml. | #Above example, Mean cell count/4 * 2 = Cells x 10<sup>4</sup>/ml. | ||
[[image:hemocytometer.png|none | [[image:hemocytometer.png|none|[[help:contents|demo]]]] | ||
==Notes== | ==Notes== |
Revision as of 10:47, 28 August 2007
Overview
Counting cells with a hemocytometer.
Materials
- Tube of cell suspension
- Hemocytometer
- Trypan Blue 0.4%
Procedure
- Combine 20μl cell suspension with 20μl of Trypan Blue. Mix.
- Load 10μl into each of the 2 hemocytometer "sides" using the capillary action created by the coverslip and the hemocytometer. Avoid bubbles.
- Count the number of cells in the gridded area of each side. (See below) White cells are live and blue cells are dead/dying.
- Record cell count and repeat for the other side of the hemocytometer.
- If the two cell counts are roughly equivalent, average the cell counts. Otherwise, recount.
- Mean cell count/4 * Dilution = Cells x 104/ml.
- Above example, Mean cell count/4 * 2 = Cells x 104/ml.
Notes
References