Sterile filters: Difference between revisions
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== Size comparison == | == Size comparison == | ||
* 0.01-0.3 µm - 10-300 nm viruses | |||
* 0.4-0.6 µm - 400-600 nm Lactobacillus brevi/Listeria innocua width [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WXV-4R5H1V8-6&_user=1592544&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000053921&_version=1&_urlVersion=0&_userid=1592544&md5=746c676e168cc76b37085d5999f199f6] | * 0.4-0.6 µm - 400-600 nm Lactobacillus brevi/Listeria innocua width [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WXV-4R5H1V8-6&_user=1592544&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000053921&_version=1&_urlVersion=0&_userid=1592544&md5=746c676e168cc76b37085d5999f199f6] | ||
* 0.7-1.2 µm - 700-1200 nm E. coli width [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WXV-4R5H1V8-6&_user=1592544&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000053921&_version=1&_urlVersion=0&_userid=1592544&md5=746c676e168cc76b37085d5999f199f6] | * 0.7-1.2 µm - 700-1200 nm E. coli width [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WXV-4R5H1V8-6&_user=1592544&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000053921&_version=1&_urlVersion=0&_userid=1592544&md5=746c676e168cc76b37085d5999f199f6] | ||
* 4-6 µm - 4000-6000 nm S. cerevisiae diameter [http://www.pnas.org/content/97/18/9871.full]) | * 4 - 6 µm - 4000-6000 nm S. cerevisiae diameter [http://www.pnas.org/content/97/18/9871.full]) | ||
== Comments == | |||
In my hands, I lose about 0.4ml in a 33mm Millex 0.22µm filter. If I squeeze through 5ml of water, the initial volume recovered is about 4.5ml. With three air washed, I can recover 4.6ml in total 0.4ml remains stuck in the filter (dead volume). --[[User:Jakob Suckale|JS]] 06:35, 25 February 2009 (EST) | |||
== Links == | == Links == | ||
* [http://www.millipore.com/techpublications/tech1/vg3561en00 Millex® 33 mm Filter Units with Durapore® PVDF Membrane - description and test] | |||
* [http://www.millipore.com/techpublications/tech1/qa13 Effect of pore size on microbrial recovery - Millipore] | * [http://www.millipore.com/techpublications/tech1/qa13 Effect of pore size on microbrial recovery - Millipore] |
Latest revision as of 04:36, 25 February 2009
Sterile filters can be used in a forward (syringe>filter>tube) and backward (tube>filter>syringe) direction to remove microorganisms and particles depending on the chosen pore size. Commonly used filters include Millipore's single use Millex filters.
Pore size
- 0.10 µm - 100 nm
- 0.22 µm - 220 nm
- 0.45 µm - 450 nm
Size comparison
- 0.01-0.3 µm - 10-300 nm viruses
- 0.4-0.6 µm - 400-600 nm Lactobacillus brevi/Listeria innocua width [1]
- 0.7-1.2 µm - 700-1200 nm E. coli width [2]
- 4 - 6 µm - 4000-6000 nm S. cerevisiae diameter [3])
Comments
In my hands, I lose about 0.4ml in a 33mm Millex 0.22µm filter. If I squeeze through 5ml of water, the initial volume recovered is about 4.5ml. With three air washed, I can recover 4.6ml in total 0.4ml remains stuck in the filter (dead volume). --JS 06:35, 25 February 2009 (EST)