Stanford/BIOE44:Module 5:Day3: Difference between revisions
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(New page: {{Template:Stanford/BioE44}} <div style="padding: 5px; width: 656px; border: 2px solid #397D02;"> =M5: Day 2 - Continue Building= ==Freezer Stocks of Synthesized Parts== '''Materials''' *...) |
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'''Procedure''' | '''Procedure''' | ||
#Label your cryovials: part name | #Label your cryovials: part name, plasmid, date, your initials. | ||
#Add 500ul of sterile 60% glycerol to each of your cryovials. | #Add 500ul of sterile 60% glycerol to each of your cryovials. | ||
#Add 1.5mL of overnight culture to each cryovial. (Its easiest to use a 5ml pipette, take up 4.5mL of culture then drop 1.5mL into each tube) | #Add 1.5mL of overnight culture to each cryovial. (Its easiest to use a 5ml pipette, take up 4.5mL of culture then drop 1.5mL into each tube) | ||
#Invert tubes several times to mix. | #Invert tubes several times to mix. | ||
#Put in freezer box in -80C freezer. (Usually one tube is kept as the working stock and the other two are kept as back ups in a separate freezer.) | #Put in freezer box in -80C freezer. (Usually one tube is kept as the working stock and the other two are kept as back ups in a separate freezer.) |
Revision as of 21:46, 22 May 2010
M5: Day 2 - Continue Building
Freezer Stocks of Synthesized Parts
Materials
- 5mL overnight culture
- sterile 60% glycerol
- 3 cryovials, labelled
Procedure
- Label your cryovials: part name, plasmid, date, your initials.
- Add 500ul of sterile 60% glycerol to each of your cryovials.
- Add 1.5mL of overnight culture to each cryovial. (Its easiest to use a 5ml pipette, take up 4.5mL of culture then drop 1.5mL into each tube)
- Invert tubes several times to mix.
- Put in freezer box in -80C freezer. (Usually one tube is kept as the working stock and the other two are kept as back ups in a separate freezer.)