Stanford/BIOE44:Module 5:Day3: Difference between revisions

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(New page: {{Template:Stanford/BioE44}} <div style="padding: 5px; width: 656px; border: 2px solid #397D02;"> =M5: Day 2 - Continue Building= ==Freezer Stocks of Synthesized Parts== '''Materials''' *...)
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Revision as of 21:45, 22 May 2010

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M5: Day 2 - Continue Building

Freezer Stocks of Synthesized Parts

Materials

  • 5mL overnight culture
  • sterile 60% glycerol
  • 3 cryovials, labelled

Procedure

  1. Label your cryovials: part name, strain, plasmid, date, your initials.
  2. Add 500ul of sterile 60% glycerol to each of your cryovials.
  3. Add 1.5mL of overnight culture to each cryovial. (Its easiest to use a 5ml pipette, take up 4.5mL of culture then drop 1.5mL into each tube)
  4. Invert tubes several times to mix.
  5. Put in freezer box in -80C freezer. (Usually one tube is kept as the working stock and the other two are kept as back ups in a separate freezer.)
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