Stanford/BIOE44:Module 5:Day1

M5: Day 1

Getting ready for parts to arrive

Parts Request

YOU MUST MAKE YOUR FINAL PARTS REQUESTS ASAP Please fill in this google doc with the information on the parts you need. In case there is overlap on the parts different groups need, there is a column for "other groups that need this part." Please fill this column in so we know how much of each part we need to make. Even if you are using a part that you think we already have, you should make a formal request.

RBS Design

A nice discussion of | RBS design can be found here.

Primer Design Guidelines

• Homology region should be about 20bp.
• Total length should be less than 100bp. Longer primers take longer to be made, so make your primers as short as possible.

Example Primer design

• Blue = Biobrick prefix or suffix
• Pink = Ribosome binding site
• Green = gene sequence (aka homology region)
• Yellow = Promoter

Adding a ribosome binding site

Forward primer: 5'-ATAGAATTCGCGGCCGCTTCTAGAGTTAAGGAGGTAAATAATGGACTATATTGTTGGTTTCG-3'

Reverse primer: 5'-ATACTGCAGCGGCCGCTACTAGTATCACCTCACTTTGGGGATAAC-3'

Adding a promoter and a ribosome binding site

Forward primer: 5'-ATAGAATTCGCGGCCGCTTCTAGAGACCAATGCTGGGAACGGCCAGGGCACCTAATTAAGGAGGTAAATAATGGACTATATTGTTGGTTTCG-3'

Reverse primer: 5'-ATACTGCAGCGGCCGCTACTAGTATCACCTCACTTTGGGGATAAC-3'

I'm Moss-ay

To do list for Tuesday (5/18):

• Finish the Moss Datasheet. Remember we're trying to make a sheet that someone could look at and understand why moss is a useful organism, what you would use it for and how. It needs to include more than what we've done in the class.
• Make BCD liquid media with mannitol.
• Look at our plates.