Stanford/BIOE44:Module 2: Difference between revisions
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| Line 39: | Line 39: | ||
| color 004 light green insert | | color 004 light green insert | ||
| digested X,P and running in gels | | digested X,P and running in gels | ||
| | | ~15uL | ||
| | | 110 ng/uL | ||
|- | |- | ||
| | | | ||
| arsR insert | | arsR insert | ||
| digested E,S and running in gels | | digested E,S and running in gels | ||
| | | ~15 uL | ||
| | | 133 ng/uL | ||
|- | |- | ||
| | | | ||
| color 004 light green insert | | color 004 light green insert | ||
| digested X,P and not running in gels yet | | digested X,P and not running in gels yet | ||
| | | 40 uL | ||
| | | 110 ng/uL | ||
|- | |- | ||
| | | | ||
| arsR | | arsR | ||
| digested E,S and not running in gels yet | | digested E,S and not running in gels yet | ||
| | | 40 uL | ||
| | | 133 ng/uL | ||
|- | |- | ||
| | | | ||
| MG1655 vector (low copy vector and red-orange insert) | | MG1655 vector (low copy vector and red-orange insert) | ||
| digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet | | digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet | ||
| | | 25uL each | ||
| | | 273 ng/uL | ||
|} | |} | ||
Revision as of 15:51, 22 April 2010
Module 2: Genetic Devices
Inventory for Arsenic Sensor
| Group Members | Part | Status | Volume | Concentration |
| Daniel and Aaditya | arsR insert | Digested at E and S | 25 uL | 36.8 ng/uL |
| arsR insert | Digested at X and S, extracted from gel | 2 gel bands | (hasn't been purified yet) | |
| Travis and Scott | none | |||
| Frank, Claire, and Sanjay | MG1655 vector | undigested | a substantial amount | 203 ng/ul |
| color 004 light green insert | digested X,P and running in gels | ~15uL | 110 ng/uL | |
| arsR insert | digested E,S and running in gels | ~15 uL | 133 ng/uL | |
| color 004 light green insert | digested X,P and not running in gels yet | 40 uL | 110 ng/uL | |
| arsR | digested E,S and not running in gels yet | 40 uL | 133 ng/uL | |
| MG1655 vector (low copy vector and red-orange insert) | digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet | 25uL each | 273 ng/uL |
Introduction
TBA
Schedule
TBA
