Stanford/BIOE44:Module 1:Day1

M1: Day 1 - Plasmid Extraction and Restriction Digest

Introduction

Before Class

• Please read:

In Class

Part 1: Plasmid extraction (aka miniprep)

At the beginning of class you will be given a 5mL culture of E. coli harboring a plasmid that contains a BioBrick part. We will extract the plasmid from the bacteria using a kit (QIAPrep Spin Miniprep kit). Then we'll measure the concentration of the plasmid DNA extracted using a nanodrop spectrophotometer.

Mini-Prep Protocol

1. Spin down culture
2. Resuspend

Part 2: Restriction Enzyme Digest

We will take the plasmid we just extracted and set up a restriction digest to cut the part out of the plasmid.