Stanford/BIOE44(S11): Assignments & Grading

Grades will be based on your performance on the following work:

50% total grade (10% per)
Lab Procedure Certifications ("BIOE.44 Certs"):
1. Preparing a -80 glycerol master stock of a clonal culture.
2. Getting useful DNA out from cells and purified.
3. Getting useful DNA into and operating inside living cells.
4. Designing and carrying out a successful PCR reaction.
5. Designing and detailing a new standard biological part.
You must complete each certification in carrying out your BIOE.44 design project. To receive credit you must submit a "cert. report" for grading. Templates will be made available.

50% total grade
BIOE.44 Design Project
Working in pairs, select a genetically encoded sensor or actuator of your own choosing, or select a short sequence of the E. coli chromosome to "refactor."
1. Describe and justify your choice in a final project report (20%)
2. Design and document and share your new standard biological parts in the Registry of Standard Biological Parts (10%)
3. Order for synthesis, receive, and test your new designer DNA (10%)
4. Organize all of your projects work into a written report, including a description of your results (10%)

5% bonus credit
Homeworks
Complete and submit short exercises due before morning class throughout the term.