Standardized GFP quantification: Difference between revisions
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==Introduction== | ==Introduction== | ||
Standardization offers many benefits when designing and characterizing engineered biological systems. While standardization can be implemented at many levels with varying levels of difficulty, a fundamental standardization is to be able to reliably. | Standardization offers many benefits when designing and characterizing engineered biological systems. While standardization can be implemented at many levels with varying levels of difficulty, a fundamental standardization is to be able to reliably quantify the number of reporter proteins per cell in a culture sample. If this quantification can be done reliably and rapidly then experimental data can be compared across experiments, instruments and labs. Ideally, a calibration curve should exist for all instruments (e.g. plate readers, flow cytometers etc.) so that the relative units on the particular machine can be related back to absolute numbers of molecules per cell. | ||
==Prior Work== | ==Prior Work== | ||
Revision as of 07:22, 25 August 2005
Contacts
Barry Canton, Caitlin Conboy, Jason Kelly, Ania Labno, Josh Michener
Introduction
Standardization offers many benefits when designing and characterizing engineered biological systems. While standardization can be implemented at many levels with varying levels of difficulty, a fundamental standardization is to be able to reliably quantify the number of reporter proteins per cell in a culture sample. If this quantification can be done reliably and rapidly then experimental data can be compared across experiments, instruments and labs. Ideally, a calibration curve should exist for all instruments (e.g. plate readers, flow cytometers etc.) so that the relative units on the particular machine can be related back to absolute numbers of molecules per cell.
