Springer Lab: Antibiotics - Revision history

Renan A. Escalante-Chong at 23:35, 18 April 2011

Renan A. Escalante-Chong — Mon, 18 Apr 2011 23:35:26 GMT

←Older revision		Revision as of 23:35, 18 April 2011
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	=G418=		=G418=
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		+	*Working concentration 200 mg/mL (1000 x)
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	http://openwetware.org/wiki/G418		http://openwetware.org/wiki/G418
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		+	=Clonat=
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		+	*Working concentration 100mg/mL (1000x)
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		+	http://openwetware.org/wiki/ClonNat

	==Experimental==		==Experimental==

Renan A. Escalante-Chong at 17:52, 21 October 2010

Renan A. Escalante-Chong — Thu, 21 Oct 2010 17:52:38 GMT

←Older revision		Revision as of 17:52, 21 October 2010
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	__NOTOC__		__NOTOC__
	Back to [[Springer Lab]]		Back to [[Springer Lab]]
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	[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).		[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).

Renan A. Escalante-Chong at 17:52, 21 October 2010

Renan A. Escalante-Chong — Thu, 21 Oct 2010 17:52:28 GMT

←Older revision		Revision as of 17:52, 21 October 2010
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	[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).		[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).

		+	=G418=
	http://openwetware.org/wiki/G418		http://openwetware.org/wiki/G418

Renan A. Escalante-Chong at 17:52, 21 October 2010

Renan A. Escalante-Chong — Thu, 21 Oct 2010 17:52:01 GMT

←Older revision		Revision as of 17:52, 21 October 2010
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	__NOTOC__		__NOTOC__
	Back to [[Springer Lab]]		Back to [[Springer Lab]]
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	[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).		[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).
		+
		+	http://openwetware.org/wiki/G418

	==Experimental==		==Experimental==

Renan A. Escalante-Chong at 17:51, 21 October 2010

Renan A. Escalante-Chong — Thu, 21 Oct 2010 17:51:24 GMT

←Older revision		Revision as of 17:51, 21 October 2010
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		+	{{Template:Springer}}
		+	__NOTOC__
		+	Back to [[Springer Lab]]
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	[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).		[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).

Renan A. Escalante-Chong: New page: Aminoglycoside antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.). ==Experimental== * '''stock solution''': 200mg/mL...

Renan A. Escalante-Chong — Thu, 21 Oct 2010 17:50:51 GMT

New page: Aminoglycoside antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.). ==Experimental== * '''stock solution''': 200mg/mL...

New page

[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).

==Experimental==

* '''stock solution''': 200mg/mL (1000X), stored at -20 C
* '''working concentration''': working concentration depends on organism and purpose of antibiotic application; stable at RT

:* yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on [[YPD]] containing 200 mg/L (=µg/ml) G418.

:* bacteria selection: 8-16 µg/ml
:* mammalian cell maintenance: 200 µg/ml
:* mammalian cell selection: 400 µg/ml (range: 300-1000 µg/ml)

==Description==

* Gentamycin analog, used to select for the KanMX marker in yeast.
* '''mode of action''': irreversibly binds to 80S ribosomal subunit, disrupting proofreading.
* '''mode of resistance''': ''neo/kan'' gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, [[neomycin]] and [[kanamycin]] by phosphorylation.

==Links==
* related antibiotics: [[kanamycin]], [[neomycin]] (all aminoglycosides)
* FAQ on G418 [http://www.g418.com/faq.htm]
* list of cell culture material by company [http://www.krackeler.com/products/fid/2910]

[[Category:Material]] [[Category:Antibiotic]]