Springer Lab: Antibiotics: Difference between revisions
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[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.). | [[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.). | ||
=G418= | |||
*Working concentration 200 mg/mL (1000 x) | |||
http://openwetware.org/wiki/G418 | http://openwetware.org/wiki/G418 | ||
=Clonat= | |||
*Working concentration 100mg/mL (1000x) | |||
http://openwetware.org/wiki/ClonNat | |||
==Experimental== | ==Experimental== |
Latest revision as of 16:35, 18 April 2011
Back to Springer Lab
Aminoglycoside antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).
G418
- Working concentration 200 mg/mL (1000 x)
http://openwetware.org/wiki/G418
Clonat
- Working concentration 100mg/mL (1000x)
http://openwetware.org/wiki/ClonNat
Experimental
- stock solution: 200mg/mL (1000X), stored at -20 C
- working concentration: working concentration depends on organism and purpose of antibiotic application; stable at RT
- yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on YPD containing 200 mg/L (=µg/ml) G418.
- bacteria selection: 8-16 µg/ml
- mammalian cell maintenance: 200 µg/ml
- mammalian cell selection: 400 µg/ml (range: 300-1000 µg/ml)
Description
- Gentamycin analog, used to select for the KanMX marker in yeast.
- mode of action: irreversibly binds to 80S ribosomal subunit, disrupting proofreading.
- mode of resistance: neo/kan gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin and kanamycin by phosphorylation.