Springer Lab: Antibiotics

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(New page: Aminoglycoside antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.). ==Experimental== * '''stock solution''': 200mg/mL...)
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[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).
[[Aminoglycoside]] antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).
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=G418=
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*Working concentration 200 mg/mL (1000 x)
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http://openwetware.org/wiki/G418
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=Clonat=
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*Working concentration 100mg/mL (1000x)
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http://openwetware.org/wiki/ClonNat
==Experimental==
==Experimental==

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Aminoglycoside antibiotic. G418/G-418 (generic name) is the same molecule as Geneticin® (registered trademark of Invitrogen Corp.).

G418

  • Working concentration 200 mg/mL (1000 x)

http://openwetware.org/wiki/G418

Clonat

  • Working concentration 100mg/mL (1000x)

http://openwetware.org/wiki/ClonNat

Experimental

  • stock solution: 200mg/mL (1000X), stored at -20 C
  • working concentration: working concentration depends on organism and purpose of antibiotic application; stable at RT
  • yeast: after transformation incubate culture at 30°C for 2-3 hours and then plate on YPD containing 200 mg/L (=µg/ml) G418.
  • bacteria selection: 8-16 µg/ml
  • mammalian cell maintenance: 200 µg/ml
  • mammalian cell selection: 400 µg/ml (range: 300-1000 µg/ml)

Description

  • Gentamycin analog, used to select for the KanMX marker in yeast.
  • mode of action: irreversibly binds to 80S ribosomal subunit, disrupting proofreading.
  • mode of resistance: neo/kan gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin and kanamycin by phosphorylation.

Links

  • related antibiotics: kanamycin, neomycin (all aminoglycosides)
  • FAQ on G418 [1]
  • list of cell culture material by company [2]
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