Springer Lab:Protocols: Difference between revisions
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===''S. cerevisiae'' Protocols=== | ===''S. cerevisiae'' Protocols=== | ||
*[http://dbb.urmc.rochester.edu/labs/sherman_f/yeast/6.html Yeast Nomenclature] | *[http://dbb.urmc.rochester.edu/labs/sherman_f/yeast/6.html Yeast Nomenclature] | ||
*[[Springer Lab: TransformationYeast | Yeast Transformation (Gietz)]] - Our standard yeast transformation protocol. | *[[Springer Lab: TransformationYeast | Yeast Transformation (Gietz)]] - Our standard yeast transformation protocol. | ||
*[[Springer Lab: TransformationYeast | Yeast Transformation (Cold Spring Harbor?)]] - a variant of the above protocol that differs in final PEG and LiAc concentrations. | *[[Springer Lab: TransformationYeast | Yeast Transformation (Cold Spring Harbor?)]] - a variant of the above protocol that differs in final PEG and LiAc concentrations. | ||
*[[Springer Lab: TransformationYeast2 | Yeast Transformation 2]] - short version of protocol | *[[Springer Lab: TransformationYeast2 | Yeast Transformation 2]] - short version of protocol | ||
*[[Springer Lab: TransformationYeast96well | Transformation Yeast 96 well]] | *[[Springer Lab: TransformationYeast96well | Transformation Yeast 96 well]] | ||
===Recipes=== | |||
*[[Springer Lab: YNB media|YNB media]] | |||
For yeast transformations | |||
*[[Springer Lab: PEG|50% PEG]] | |||
==Antibiotics== | ==Antibiotics== | ||
Revision as of 15:51, 15 April 2011
Protocols
S. cerevisiae Protocols
- Yeast Nomenclature
- Yeast Transformation (Gietz) - Our standard yeast transformation protocol.
- Yeast Transformation (Cold Spring Harbor?) - a variant of the above protocol that differs in final PEG and LiAc concentrations.
- Yeast Transformation 2 - short version of protocol
- Transformation Yeast 96 well
Recipes
For yeast transformations
