User contributions
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- 11:58, 30 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-30
- 11:44, 30 July 2007 (hist) (diff) N Registry/Measurement kit/Notebook/2007-7-30 (New page: ==To Do== *See about making competent DB3.1 cells *Test the top10 stock with a + control (pUC18) *See what we can do with BB'd E0240 & I2057 ==Promoter Testers== *Should pick some promote...)
- 10:27, 30 July 2007 (hist) (diff) Nm Registry/Measurement kit/Notebook/2007-7-29 (New page: *Prepared sequencing reactions for BB'd devices and these build attempts: **I2056-3k3 (2 samples) **I2055-3k3 (2 samples) **E0240-1AK3 (1 sample) *Replated transformations from yesterday) (top)
- 10:24, 30 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-28 (top)
- 10:24, 30 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-28
- 10:23, 30 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-28
- 10:22, 30 July 2007 (hist) (diff) N Image:7-28-07-sm.jpg (top)
- 10:21, 30 July 2007 (hist) (diff) N Image:7-28-lrg.jpg (top)
- 11:19, 29 July 2007 (hist) (diff) N Registry/Measurement kit/Notebook/2007-7-28 (New page: ==Overnight cultures== *Growth on: **E0240-1AK3 **I2055-1AK3 **I2057-1AK3 **I2056-1AO3 **Both promoter testers (3K3, ligated at NotI) versions E-X and E-S *None: **F2620-1AK3 and 3K3 **Re...)
- 16:02, 27 July 2007 (hist) (diff) N Registry/Measurement kit/Notebook/2007-7-27 (New page: *Re-ligated digests: I2057, E0240, and F2620 in 3K3 and 1AK3 *Transformed and plated) (top)
- 15:59, 27 July 2007 (hist) (diff) m Endy:Pouring LB plates from prepped media (→Actual Pouring)
- 17:47, 26 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-26 (top)
- 17:37, 26 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-26
- 17:29, 26 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-26
- 17:28, 26 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-26
- 17:27, 26 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-26
- 10:35, 26 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-26 (→Plates)
- 10:32, 26 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-26 (→To Do)
- 10:32, 26 July 2007 (hist) (diff) N Registry/Measurement kit/Notebook/2007-7-26 (New page: ==Plates== *No growth on F2620-3K3 sample 2 or I2056. *F2620-3K3 sample 1 and F2620-1AK3 have a few small colonies. ==To Do== *PCR clean digests from yesterday *Ligate them *Mini-Prep and...)
- 16:05, 25 July 2007 (hist) (diff) N Registry/Measurement kit/Notebook/2007-7-22 (New page: *PCR'd 8 colonies of I2055, only #2 looked right size.) (top)
- 16:02, 25 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-23 (top)
- 15:56, 25 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-25 (→T9002) (top)
- 15:53, 25 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-25
- 15:33, 25 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-25
- 14:52, 25 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-25
- 14:40, 25 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-25
- 14:39, 25 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-25 (→Misc)
- 13:52, 25 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-25
- 13:47, 25 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-25
- 12:12, 25 July 2007 (hist) (diff) N Registry/Measurement kit/Notebook/2007-7-25 (New page: ==T9002== *F2620 was ligated into 3K3(E/P) instead of E/X *Re-ligated F2620 (M/X) into 3K3 (E/X) and 1AK3 (E/X) ==Misc== *<s>Also Digesting more 3K3 (E/P)</s> Making o/n of 3K3 *Mini-prep...)
- 16:52, 24 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-24 (top)
- 16:52, 24 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-24
- 16:03, 24 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-24
- 11:48, 24 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-24
- 11:28, 24 July 2007 (hist) (diff) N Registry/Measurement kit/Notebook/2007-7-24 (New page: *Ran gel of I2056, got a very strong band.)
- 11:27, 24 July 2007 (hist) (diff) N Registry/Measurement kit/Notebook/2007-7-23 (New page: *Sent in final order for BB primers. **Ran gradient PCR of I2056 w/promoter (50-57) with the supermix, all bands look ok. *Started overnight PCR at 51 deg)
- 14:30, 23 July 2007 (hist) (diff) N Endy:PCR Supermix (New page: For a 100µL reaction using the supermix: #100µL PCR Supermix #1.5µL forward primer (40 mM) #1.5µL reverse primer (40 mM) #0.5µL template DNA (10ng/µL) Protocol: #95 deg - 5 min #95...) (top)
- 14:25, 23 July 2007 (hist) (diff) User:Nishant M. Bhat/Protocols (top)
- 15:22, 20 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-20 (top)
- 03:21, 20 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-19 (→Primer order) (top)
- 03:17, 20 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-19
- 03:16, 20 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-19
- 03:16, 20 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-19 (→Primer order)
- 18:26, 19 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-19
- 18:25, 19 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-19
- 16:56, 19 July 2007 (hist) (diff) User:Jessica Karen Wong/Primers (→BioBrick Promers)
- 16:50, 19 July 2007 (hist) (diff) User:Jessica Karen Wong/Primers (→BioBrick Promers)
- 14:25, 19 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-19
- 00:47, 18 July 2007 (hist) (diff) Registry/Measurement kit/Notebook/2007-7-17 (top)
- 12:34, 17 July 2007 (hist) (diff) N Registry/Measurement kit/Notebook/2007-7-17 (New page: Prepped, sent colonies for sequencing: #528f #528r #507f #507r #I2055 1AK3 #4 (VF2) #I2055 1AK3 #4 (VR) #E0240 3K3 #9 (VF) #E0240 3K3 #9 (VR) #E0240 1AK3 #1 (VF) #E0240 1AK3 #1 (VR) #E0...)
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