User contributions
From OpenWetWare
(Latest | Earliest) View (newer 50) (older 50) (20 | 50 | 100 | 250 | 500)
- 15:00, 26 April 2007 (hist) (diff) McKinney:MaterialsAndMethods (→Software)
- 17:32, 17 April 2007 (hist) (diff) m McKinney:7H9
- 17:32, 17 April 2007 (hist) (diff) N McKinney:SodiumOleate (New page: ===Sodium oleate solution=== This is an additive to media used to culture ''M. tuberculosis.'' ===Ingredients=== (make 500mL) * 12.5mL 2M NaOH * 6.0mL oleic acid (=5g, since oleic acid...) (top)
- 17:30, 17 April 2007 (hist) (diff) N McKinney:OADC (New page: ===Summary=== OADC is Oleic Acid Albumin Dextrose Complex, a component of the mycobacterial selective media 7H9 and 7H10. If you don't add the oleic acid solution, then this is ADS, used...) (top)
- 17:26, 17 April 2007 (hist) (diff) m McKinney:7H10 (top)
- 17:25, 17 April 2007 (hist) (diff) N McKinney:7H10 (New page: ===Summary=== 7H10 agar is selective medium used for the culture of mycobacteria, in particular ''M. tuberculosis.'' ''M. smegmatis'' is usually grown on LB agar. ===Ingredients=== * 1...)
- 17:16, 17 April 2007 (hist) (diff) McKinney:7H9
- 17:00, 17 April 2007 (hist) (diff) N McKinney:7H9 (New page: ===Summary=== 7H9 (and its solid counterpart, 7H10 agar) are selective media used for the culture of free-living mycobacteria such as ''M. tuberculosis'' and ''M. smegma...)
- 16:51, 17 April 2007 (hist) (diff) m Materials (→Standard Media)
- 16:47, 17 April 2007 (hist) (diff) Materials (→Standard Media)
- 10:44, 8 April 2007 (hist) (diff) Madhadron:Cruft
- 15:03, 3 April 2007 (hist) (diff) Madhadron:ChromosomeMarker
- 11:35, 28 March 2007 (hist) (diff) Madhadron:Cruft (→Ligating DNA)
- 15:35, 27 March 2007 (hist) (diff) Madhadron:Cruft
- 11:25, 27 March 2007 (hist) (diff) Madhadron:ChromosomeMarker
- 18:52, 24 March 2007 (hist) (diff) Madhadron:Cruft (→DNA purification)
- 18:50, 24 March 2007 (hist) (diff) Madhadron:Cruft (→DNA purification)
- 18:43, 24 March 2007 (hist) (diff) Madhadron:Cruft (→DNA purification)
- 18:42, 24 March 2007 (hist) (diff) Maxiprep of plasmid DNA from E. coli
- 18:41, 24 March 2007 (hist) (diff) Miniprep/Kit-free high-throughput protocol
- 13:48, 23 March 2007 (hist) (diff) m Madhadron:PreparingCellsForMicroscope
- 13:47, 23 March 2007 (hist) (diff) User:Madhadron (→Bench Stuff)
- 13:35, 23 March 2007 (hist) (diff) N Madhadron:PreparingCellsForMicroscope (New page: ===Materials=== * 1mL sterile syringe (BD 1mL tuberculin slip tip) * 5μm filter (Millex-SV, fits onto end of syringe) ===Directions=== # Take a culture of ''M. smegmatis'' in exponenti...)
- 13:29, 23 March 2007 (hist) (diff) User:Madhadron (→Protocols in development)
- 12:01, 23 March 2007 (hist) (diff) Madhadron:Cruft
- 11:38, 23 March 2007 (hist) (diff) Madhadron:Cruft
- 22:44, 20 March 2007 (hist) (diff) m Madhadron:TmeMonitor (top)
- 22:37, 20 March 2007 (hist) (diff) N Image:Tmemonitor-20070320.tgz (CGI scripts for monitoring temperatures with a Papouch ethernet thermometer, written in McKinney lab by Fred Ross.) (top)
- 22:36, 20 March 2007 (hist) (diff) N Madhadron:TmeMonitor (New page: McKinney Lab uses an ethernet thermometer from [http://www.papouch.com Papouch] to monitor the temperature of our shaking incubator. I have written some simple CGI code to re...)
- 22:31, 20 March 2007 (hist) (diff) McKinney:MaterialsAndMethods (→Software)
- 19:16, 17 March 2007 (hist) (diff) Rifampicin
- 19:07, 17 March 2007 (hist) (diff) m Maxiprep of plasmid DNA from E. coli (→Ingredients)
- 19:07, 17 March 2007 (hist) (diff) m Maxiprep of plasmid DNA from E. coli (→Ingredients)
- 19:06, 17 March 2007 (hist) (diff) Maxiprep of plasmid DNA from E. coli (→Ingredients)
- 18:30, 14 March 2007 (hist) (diff) N Madhadron:DICOnAgar (New page: From Gilles: Put 2mL of 2% agar (liquid) on a slide, and put a cover slip over it. Let cook, slide coverslip off. Cut little square of agar out with scalpel. Apply cells to top of it, t...)
- 18:29, 14 March 2007 (hist) (diff) User:Madhadron (→Protocols in development)
- 16:25, 9 March 2007 (hist) (diff) McKinney:MaterialsAndMethods
- 15:30, 9 March 2007 (hist) (diff) Madhadron:Preprocessor (top)
- 15:29, 9 March 2007 (hist) (diff) N Image:Mycopreprocesser-20070309.jar (Jar file for the McKinney Lab image stack preprocessor.) (top)
- 15:28, 9 March 2007 (hist) (diff) m Madhadron:Preprocessor
- 15:27, 9 March 2007 (hist) (diff) N Madhadron:Preprocessor (New page: The jar file preprocessor-[date].jar below is the latest release of the preprocessor we're using for our image analysis. It has two stages. The first registers the image stack. The seco...)
- 15:16, 9 March 2007 (hist) (diff) McKinney:MaterialsAndMethods
- 13:15, 23 February 2007 (hist) (diff) Madhadron:Cruft
- 16:58, 22 February 2007 (hist) (diff) Madhadron:Cruft
- 11:31, 15 February 2007 (hist) (diff) Maxiprep of plasmid DNA from E. coli
- 15:12, 13 February 2007 (hist) (diff) McKinney:MaterialsAndMethods (→Protocols)
- 15:04, 13 February 2007 (hist) (diff) Maxiprep of plasmid DNA from E. coli
- 15:04, 13 February 2007 (hist) (diff) N Maxiprep of plasmid DNA from E. coli (New page: This protocol produces very clean, concentrated DNA, usually on the order of several mg/mL. It is labor intensive. Expect to spend most of the day doing it, but I like to store my plasmi...)
- 14:59, 13 February 2007 (hist) (diff) Protocols (→''In vivo'')
- 13:43, 13 February 2007 (hist) (diff) LabbookInLatex (top)
(Latest | Earliest) View (newer 50) (older 50) (20 | 50 | 100 | 250 | 500)


