Sortostat/Setting up a new chip

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Revision as of 10:28, 11 January 2006 by Jason R. Kelly (talk | contribs) (SortoStat/Setting up a new chip moved to Sortostat/Setting up a new chip)
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this is a work in progress

<need a diagram of the setup here>

Materials

Filter-sterilzed (200nm) ddH20

Moving tubing from an old chip to a fresh one

  1. Remove pins 1-8 from the old chip
  2. Detach tubes 1-8 from the tubes connected to the manifold (do not remove the tubes from the manifold itself).
    • The advatage of this is that you can now remove the tubing from the incubator and it is much easier to work with.
  3. Insert a syringe with a luer adapter into the end of tube#1 and fill the tube with water. Use the dripping water to identify the pin #1 and insert it into the appropriate position on the chip.
    • Repeat this for tubes 2-8.
  4. When 1-8 have been filled and attached move onto the next block of 8 and repeat for all 32 control tubes.


Contact:Jason Kelly