Sortostat/Experiments/Two reactor chip: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 48: | Line 48: | ||
===7/11/07b=== | ===7/11/07b=== | ||
*cleaning period = 14.8 | *cleaning period = 14.8 | ||
*Focus: 1375, 1984, 2102, 3648, 4068, 5600, 6022, 7502, 8008, 9100, 9718, 9927, 11062, 13036 | *Focus: 1375, 1984, 2102, 3648, 4068, 5600, 6022, 7502, 8008, 9100, 9718, 9927, 11062, 13036, 13638 | ||
*1436 - Started sorting againstYFP | *1436 - Started sorting againstYFP | ||
*9927 - turned off sorting | *9927 - turned off sorting | ||
Revision as of 12:54, 18 July 2007
This is a summary of successful experiments done with the new version of the chip (two reactors).
Feb2006
- What was the date of this experiment
- post image
4/21/07
- post images of %CFP and cell counts
5/29/07
- Media:
- 0.4% glycerol
- Amp
- BSA(2mg/ml)
- Temp: 37C (set to 40.4 on the incubator).
- What was the offset?
- Fluid: 5.1 psi
- Push down: 21.9 psi
- Push up: 20.2 psi
- Clean period:15.9 min
- of sorts / clean: 10
- Number of screens / sort: 2
- Cleaning block length (min): 1
- Pump cycle period (ms): 400
- Ratio history length: 15
- Pump period between screens (sec): 5
- Wash sorting chamber period (ms): 1000
- Media inlet source: center
- Feeder fill period (ms): 1000
- Clean period (ms): 15000
- Pressurize period (ms): 1000
Refocus
- 4465, 4756, 6863, 8375, 9924, 10643, 10814, 11581, 12265, 13087
Flips
- 4888
- 6864 - against YFP
- 9068 - against CFP
- 10324 - against YFP
- 11586 - random sorting
- 13087 - against CFP
7/11/07
7/11/07
- cleaning period = 15.9
7/11/07b
- cleaning period = 14.8
- Focus: 1375, 1984, 2102, 3648, 4068, 5600, 6022, 7502, 8008, 9100, 9718, 9927, 11062, 13036, 13638
- 1436 - Started sorting againstYFP
- 9927 - turned off sorting
