Sortostat/Experiments/Early experiments
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Early Results
This 2 hours at dilution rate of ~.7 hr-1 (~1hr doubling time).
Experiment on 06/07/2005
Experimental Conditions
- Strain: MC4100
- Device: I7101
- Media: Supplemented M9 + Amp
- Temp: 37C
Chemostat Operating Conditions
- Dilution Rate: 0.3 hr-1
- Clean Chemostat Period: 5hr
- Minimum Pumping Time: 150s
- Dead Time per Screen: 10s
- Dead Time per Sort: 40s
- Pump Cycle Period: 400s
- Actual Cleaning Period: 4.9333hr
- Sorting Perdiod: 6.1667 m
- Feeder Fill: 10s
- Wash: 10s
- Clean: 15s
- Pressurize: 5s
- Fluid Pressure: 5.2 psi
- Push-UP: 15.6 psi
- Push-DOWN: 16.7 psi
Results
Looks like hit SS @ ~15,000 cells in the reactor volume (10nL). This is 1.5*10^9 cells/ml.
Experiment on 6/9 to 6/13/05
Experimental Conditions
- Strain: MC4100
- Device: I7101
- Media: Supplemented M9 + Amp
- Temp: 37C
Chemostat Operating Conditions
- Same as 6/7 experiment.
- Except dilution rate varied from D=0.3 to 0.6 and the min pump period for D=0.6 was set to 50s. Cleaning period and sorting period varied for each dilution rate.
Results
Darker lines are moving average with 10 time point window.
Looked pretty good. Probably should have left longer at D=0.5 (first time) to see where the SS was exactly since it looks like the cell count may still have been decreasing. Cell counts were determined by processing of FL images as shown here.
Experiment 8/8/1005 to
Experimental Conditions for GRwoth Test
- Strain: MC4100
- Device: I13602,
- Media: Supplemented M9 + Amp
- Temp: 37C
Dilution Rate: 0.05
- Clean Chemostat Period: 30hr
- Minimum Pumping Time: 300s
- Dead Time per Screen: 10s
- Dead Time per Sort: 40s
- Pump Cycle Period: 400s
- Actual Cleaning Period: 29.6hr
- Sorting Perdiod: 37min
- Feeder Fill: 10s
- Wash: 10s
- Clean: 15s
- Pressurize: 5s
- Fluid Pressure: 5.8 psi
- Push-UP: 15 psi
- Push-DOWN: 15 psi
