Sortostat/Experiments/Early experiments: Difference between revisions
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===Experimental Conditions=== | ===Experimental Conditions=== | ||
*Strain: [[Genotypes#MC4100|MC4100]] | *Strain: [[Genotypes#MC4100|MC4100]] | ||
*Device: | *Device: I7101 | ||
*Media: [[M9 supplemented media|Supplemented M9]] + [[Ampicillin|Amp]] | *Media: [[M9 supplemented media|Supplemented M9]] + [[Ampicillin|Amp]] | ||
*Temp: 37C | *Temp: 37C | ||
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[[Image:050607Chemo12hr.PNG|Chemostat Run]] | [[Image:050607Chemo12hr.PNG|Chemostat Run]] | ||
Looks | Looks like hit SS @ ~15,000 cells in the reactor volume (10pL). This is 1.5*10^12 cells/ml. (alot) | ||
[[Sortostat Image Processing]] | [[Sortostat Image Processing]] | ||
== Experiment on 6/9 to 6/13/05 == | |||
===Experimental Conditions=== | |||
*Strain: [[Genotypes#MC4100|MC4100]] | |||
*Device: I7101 | |||
*Media: [[M9 supplemented media|Supplemented M9]] + [[Ampicillin|Amp]] | |||
*Temp: 37C | |||
===Chemostat Operating Conditions=== | |||
*Same as [[SortoStat#Experiment on 06/07/2005|6/7 experiment]]. | |||
**Except dilution rate varied from D=0.3 to 0.6 and the min pump period for D=0.6 was set to 50s. Cleaning period and sorting period varied for each dilution rate. | |||
===Results=== | |||
[[Image:90hrRunf.PNG]] |
Revision as of 10:27, 13 June 2005
Early Results
This 2 hours at dilution rate of ~.7 hr-1 (~1hr doubling time).
Experiment on 06/07/2005
Experimental Conditions
- Strain: MC4100
- Device: I7101
- Media: Supplemented M9 + Amp
- Temp: 37C
Chemostat Operating Conditions
- Dilution Rate: 0.3 hr-1
- Clean Chemostat Period: 5hr
- Minimum Pumping Time: 150s
- Dead Time per Screen: 10s
- Dead Time per Sort: 40s
- Pump Cycle Period: 400s
- Actual Cleaning Period: 4.9333hr
- Sorting Perdiod: 6.1667 m
- Feeder Fill: 10s
- Wash: 10s
- Clean: 15s
- Pressurize: 5s
- Fluid Pressure: 5.2 psi
- Push-UP: 15.6 psi
- Push-DOWN: 16.7 psi
Results
Looks like hit SS @ ~15,000 cells in the reactor volume (10pL). This is 1.5*10^12 cells/ml. (alot)
Experiment on 6/9 to 6/13/05
Experimental Conditions
- Strain: MC4100
- Device: I7101
- Media: Supplemented M9 + Amp
- Temp: 37C
Chemostat Operating Conditions
- Same as 6/7 experiment.
- Except dilution rate varied from D=0.3 to 0.6 and the min pump period for D=0.6 was set to 50s. Cleaning period and sorting period varied for each dilution rate.