Sortostat/Experiments/Early experiments: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 35: Line 35:
[[Image:050607Chemo12hr.PNG|Chemostat Run]]  
[[Image:050607Chemo12hr.PNG|Chemostat Run]]  


 
Looks liek hit SS @ ~15,000 cells in the reactor volume (10pL).  This is 1.5*10^12 cells/ml.  (alot)


[[Sortostat Image Processing]]
[[Sortostat Image Processing]]

Revision as of 14:01, 8 June 2005

Early Results

This 2 hours at dilution rate of ~.7 hr-1 (~1hr doubling time).

Early Steady-stateish results

Experiment on 06/07/2005

Experimental Conditions

Chemostat Operating Conditions

  • Dilution Rate: 0.3 hr-1
  • Clean Chemostat Period: 5hr
  • Minimum Pumping Time: 150s
  • Dead Time per Screen: 10s
  • Dead Time per Sort: 40s
  • Pump Cycle Period: 400s


  • Actual Cleaning Period: 4.9333hr
  • Sorting Perdiod: 6.1667 m


  • Feeder Fill: 10s
  • Wash: 10s
  • Clean: 15s
  • Pressurize: 5s

Results

Chemostat Run

Looks liek hit SS @ ~15,000 cells in the reactor volume (10pL). This is 1.5*10^12 cells/ml. (alot)

Sortostat Image Processing