Sortostat/Experiments/Brian growth tests

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03/16/06

Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

  • Doubling Time: Want:1hr Get:55.45min

Chemostat Settings

  • There are no sorts in this run.
  • Flow Rate: 11.09nL/hr
    • Actual: 12.12nL/hr
  • Dilution Rate: 0.69/hr
    • Actual: 0.75/hr
  • "Clean Chemostat Max Period": 1.44928hr
    • "Actual": 1.32hr

"Dilution Rate": 1/hr

  • Minimum Pumping Time: 30s
  • Dead Time per Screen: 20s
  • Dead Time per Sort: 47s
  • Pump Cycle Period: 400ms
  • Sorting Perdiod: 4.95
  • Feeder Fill: 10s
  • Wash: 10s
  • Clean: 15s
  • Pressurize: 5s
  • Fluid Pressure: 5.07 psi
  • Push-UP: 14.07 psi
  • Push-DOWN: 20.02 psi

Results

03/12/06

Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

Chemostat Settings

  • There are no sorts in this run.
  • Dilution Rate: .69/hr
  • Clean Chemostat Period: 1.45hr
  • Minimum Pumping Time: 30s
  • Dead Time per Screen: 20s
  • Dead Time per Sort: 47s
  • Pump Cycle Period: 400ms
  • Actual Cleaning Period: 1.32hr
  • Sorting Perdiod: 4.95
  • Feeder Fill: 10s
  • Wash: 10s
  • Clean: 15s
  • Pressurize: 5s
  • Fluid Pressure: 4.47 psi
  • Push-UP: 13.91 psi
  • Push-DOWN: 19.7 psi

Results

Catastrophic Failure! Airline blew off actuator and all tank pressure was lost.

Cells look to stabilize at ~100, then the pressure fails and cells diverge from steady state


01/24/06

Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

Chemostat Settings

  • There are no sorts in this run.
  • Dilution Rate: Varies, (see graph)
  • Clean Chemostat Period: 1/D.R.
  • Minimum Pumping Time: Changed from 60s to 50s when the dilution rate changed from 0.4/hr to 0.5/hr
  • Dead Time per Screen: 10s
  • Dead Time per Sort: 75s
  • Pump Cycle Period: 400ms
  • Actual Cleaning Period: ?
  • Sorting Perdiod: ?
  • Feeder Fill: 10s
  • Wash: 10s
  • Clean: 15s
  • Pressurize: 5s
  • Fluid Pressure: 5.19 psi
  • Push-UP: 15.47 psi
  • Push-DOWN: 19.39 psi

Results

Equipment failure

Previous Work

10/09/05

Cell death in long term experiment due to possible O2 depletion
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