Sortostat/Experiments/Brian growth tests: Difference between revisions
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==03/16/06== | |||
'''Purpose:''' To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation. | |||
===Experimental Conditions=== | |||
*Strain: [[Genotypes#|MC4100]] | |||
*Device: [http://parts.mit.edu/r/parts/partsdb/view.cgi?part_id=4465 I6032] | |||
*Media: [[M9 supplemented media|Supplemented M9]] + [[Ampicillin|Amp]] | |||
*Temp: 37C | |||
===Chemostat Settings=== | |||
*''There are no sorts in this run. | |||
*Flow Rate: 11.09nL/hr | |||
**Actual: 12.12nL/hr | |||
*Dilution Rate: 0.69/hr | |||
**Actual: 0.75/hr | |||
*"Clean Chemostat Max Period": 1.44928hr | |||
**"Actual": 1.32hr | |||
"Dilution Rate": 1/hr | |||
*Minimum Pumping Time: 30s | |||
*Dead Time per Screen: 20s | |||
*Dead Time per Sort: 47s | |||
*Pump Cycle Period: 400ms | |||
*Sorting Perdiod: 4.95 | |||
*Feeder Fill: 10s | |||
*Wash: 10s | |||
*Clean: 15s | |||
*Pressurize: 5s | |||
*Fluid Pressure: 5.07 psi | |||
*Push-UP: 14.07 psi | |||
*Push-DOWN: 20.02 psi | |||
===Results=== | |||
==03/12/06== | ==03/12/06== | ||
'''Purpose:''' To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation. | '''Purpose:''' To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation. | ||
Line 9: | Line 44: | ||
===Chemostat Settings=== | ===Chemostat Settings=== | ||
''There are no sorts in this run.'' | *''There are no sorts in this run.'' | ||
*Dilution Rate: .69/hr | *Dilution Rate: .69/hr | ||
*Clean Chemostat Period: 1.45hr | *Clean Chemostat Period: 1.45hr | ||
Line 44: | Line 79: | ||
===Chemostat Settings=== | ===Chemostat Settings=== | ||
''There are no sorts in this run.'' | *''There are no sorts in this run.'' | ||
*Dilution Rate: Varies, (see graph) | *Dilution Rate: Varies, (see graph) | ||
*Clean Chemostat Period: 1/D.R. | *Clean Chemostat Period: 1/D.R. |
Revision as of 19:07, 16 March 2006
03/16/06
Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.
Experimental Conditions
- Strain: MC4100
- Device: I6032
- Media: Supplemented M9 + Amp
- Temp: 37C
Chemostat Settings
- There are no sorts in this run.
- Flow Rate: 11.09nL/hr
- Actual: 12.12nL/hr
- Dilution Rate: 0.69/hr
- Actual: 0.75/hr
- "Clean Chemostat Max Period": 1.44928hr
- "Actual": 1.32hr
"Dilution Rate": 1/hr
- Minimum Pumping Time: 30s
- Dead Time per Screen: 20s
- Dead Time per Sort: 47s
- Pump Cycle Period: 400ms
- Sorting Perdiod: 4.95
- Feeder Fill: 10s
- Wash: 10s
- Clean: 15s
- Pressurize: 5s
- Fluid Pressure: 5.07 psi
- Push-UP: 14.07 psi
- Push-DOWN: 20.02 psi
Results
03/12/06
Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.
Experimental Conditions
- Strain: MC4100
- Device: I6032
- Media: Supplemented M9 + Amp
- Temp: 37C
Chemostat Settings
- There are no sorts in this run.
- Dilution Rate: .69/hr
- Clean Chemostat Period: 1.45hr
- Minimum Pumping Time: 30s
- Dead Time per Screen: 20s
- Dead Time per Sort: 47s
- Pump Cycle Period: 400ms
- Actual Cleaning Period: 1.32hr
- Sorting Perdiod: 4.95
- Feeder Fill: 10s
- Wash: 10s
- Clean: 15s
- Pressurize: 5s
- Fluid Pressure: 4.47 psi
- Push-UP: 13.91 psi
- Push-DOWN: 19.7 psi
Results
Catastrophic Failure! Airline blew off actuator and all tank pressure was lost.
01/24/06
Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.
Experimental Conditions
- Strain: MC4100
- Device: I6032
- Media: Supplemented M9 + Amp
- Temp: 37C
Chemostat Settings
- There are no sorts in this run.
- Dilution Rate: Varies, (see graph)
- Clean Chemostat Period: 1/D.R.
- Minimum Pumping Time: Changed from 60s to 50s when the dilution rate changed from 0.4/hr to 0.5/hr
- Dead Time per Screen: 10s
- Dead Time per Sort: 75s
- Pump Cycle Period: 400ms
- Actual Cleaning Period: ?
- Sorting Perdiod: ?
- Feeder Fill: 10s
- Wash: 10s
- Clean: 15s
- Pressurize: 5s
- Fluid Pressure: 5.19 psi
- Push-UP: 15.47 psi
- Push-DOWN: 19.39 psi
Results
Equipment failure