Sortostat/Experiments/Brian growth tests: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 29: Line 29:


===Results===
===Results===
Catastrophic Failure!  Airline blew off actuator and all tank pressure was lost.


==01/24/06==
==01/24/06==

Revision as of 17:46, 16 March 2006

03/12/06

Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

Chemostat Settings

  • There are no sorts in this run.
  • Dilution Rate: .69/hr
  • Clean Chemostat Period: 1.45hr
  • Minimum Pumping Time: 30s
  • Dead Time per Screen: 20s
  • Dead Time per Sort: 47s
  • Pump Cycle Period: 400ms
  • Actual Cleaning Period: 1.32hr
  • Sorting Perdiod: 4.95
  • Feeder Fill: 10s
  • Wash: 10s
  • Clean: 15s
  • Pressurize: 5s
  • Fluid Pressure: 4.47 psi
  • Push-UP: 13.91 psi
  • Push-DOWN: 19.7 psi

Results

Catastrophic Failure! Airline blew off actuator and all tank pressure was lost.

01/24/06

Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

Chemostat Settings

  • There are no sorts in this run.
  • Dilution Rate: Varies, (see graph)
  • Clean Chemostat Period: 1/D.R.
  • Minimum Pumping Time: Changed from 60s to 50s when the dilution rate changed from 0.4/hr to 0.5/hr
  • Dead Time per Screen: 10s
  • Dead Time per Sort: 75s
  • Pump Cycle Period: 400ms
  • Actual Cleaning Period: ?
  • Sorting Perdiod: ?
  • Feeder Fill: 10s
  • Wash: 10s
  • Clean: 15s
  • Pressurize: 5s
  • Fluid Pressure: 5.19 psi
  • Push-UP: 15.47 psi
  • Push-DOWN: 19.39 psi

Results

Equipment failure

Previous Work

10/09/05

Cell death in long term experiment due to possible O2 depletion