Sortostat/Experiments/Brian growth tests: Difference between revisions

Revision as of 16:09, 13 March 2006

Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

There are no sorts in this run.
Dilution Rate: Varies, (see graph)
Clean Chemostat Period: 1/D.R.
Minimum Pumping Time: Changed from 60s to 50s when the dilution rate changed from 0.4/hr to 0.5/hr
Dead Time per Screen: 10s
Dead Time per Sort: 75s
Pump Cycle Period: 400ms

Equipment failure

@@ Line 1: / Line 1: @@
+==03/12/06==
+'''Purpose:''' To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.
+===Experimental Conditions===
+*Strain: [[Genotypes#|MC4100]]
+*Device: [http://parts.mit.edu/r/parts/partsdb/view.cgi?part_id=4465 I6032]
+*Media: [[M9 supplemented media|Supplemented M9]] + [[Ampicillin|Amp]]
+*Temp: 37C
+===Chemostat Settings===
+*''There are no sorts in this run.''
+*Dilution Rate: .69/hr
+*Clean Chemostat Period: 1.45hr
+*Minimum Pumping Time: 30s
+*Dead Time per Screen: 20s
+*Dead Time per Sort: 47s
+*Pump Cycle Period: 400ms
+*Actual Cleaning Period: 1.32hr
+*Sorting Perdiod: 4.95
+*Feeder Fill: 10s
+*Wash: 10s
+*Clean: 15s
+*Pressurize: 5s
+*Fluid Pressure: 4.47 psi
+*Push-UP: 13.91 psi
+*Push-DOWN: 19.7 psi
+===Results===
 ==01/24/06==
 '''Purpose:''' To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.