Sortostat/Experiments/Brian growth tests: Difference between revisions
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===Experimental Conditions=== | ===Experimental Conditions=== | ||
*Strain: [[Genotypes#|MC4100]] | *Strain: [[Genotypes#|MC4100]] | ||
*Device: I6032 | *Device: [http://parts.mit.edu/r/parts/partsdb/view.cgi?part_id=4465 I6032] | ||
*Media: Supplemented M9 + Amp | *Media: Supplemented M9 + Amp | ||
*Temp: 37C | *Temp: 37C |
Revision as of 10:56, 25 January 2006
01/24/06
Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.
Experimental Conditions
Chemostat Settings
- There are no sorts in this run.
- Dilution Rate: Varies, (see graph)
- Clean Chemostat Period: 1/D.R.
- Minimum Pumping Time: Changed from 60s to 50s when the dilution rate changed from 0.4/hr to 0.5/hr
- Dead Time per Screen: 10s
- Dead Time per Sort: 75s
- Pump Cycle Period: 400ms
- Actual Cleaning Period: ?
- Sorting Perdiod: ?
- Feeder Fill: 10s
- Wash: 10s
- Clean: 15s
- Pressurize: 5s
- Fluid Pressure: 5.19 psi
- Push-UP: 15.47 psi
- Push-DOWN: 19.39 psi