This section describes common failure points to help in debugging.
- Apply too high of a dilution rate too quickly
- usually a good idea to grow the cells with a very low dilution rate (e.g. D=0.05) for ~10hrs in order to ensure that the cells are growing well and that there are no problems with the chip. If the cells grow well then you can increase the dilution rate.
- Cells escaping from the sorting chamber
- This can happen as a result of closing both the CW and CCW valves, resulting in a pressure build up in the chambers between push-UP valves. It is not clear how pervasive a problem this is, but I have made changes to the Microfluidic chemostat labview program to close the CW and CCW sequentially to help the problem.
- Cells stop producing fluorescent proteins