SortoStat/Operation - Revision history

Reshma P. Shetty at 17:56, 9 May 2007

2007-05-09T17:56:49Z

←Older revision		Revision as of 17:56, 9 May 2007
Line 101:		Line 101:

	''Contact:'' [[Jason Kelly]]		''Contact:'' [[Jason Kelly]]
		+
		+	[[Category:Protocol]]
		+	[[Category:Microfluidics]]

Jason R. Kelly: /* Test the device operation */

2006-01-20T17:05:31Z

Test the device operation

←Older revision		Revision as of 17:05, 20 January 2006
Line 48:		Line 48:

	===Test the device operation===		===Test the device operation===
		+
	#Pump		#Pump
	#*Test if the cells move well in response to the pump		#*Test if the cells move well in response to the pump

Jason R. Kelly: /* Test the device operation */

2006-01-20T16:34:03Z

Test the device operation

←Older revision		Revision as of 16:34, 20 January 2006
Line 55:		Line 55:
	#Screening event		#Screening event
	#*confirm that the images in the 2 debug windows show up (both FL and BF). If they don't try restarting the camera. Also in the device section of 'my computer' in windows you can check if the camera is working properly.		#*confirm that the images in the 2 debug windows show up (both FL and BF). If they don't try restarting the camera. Also in the device section of 'my computer' in windows you can check if the camera is working properly.
		+	#*If you are getting a "another program is using this device error" when you click on camera in My Computer, you may wnt to stop the LabView VI (don't close entire program!) you are currently running and try again.
	#Sorting event		#Sorting event
	#*There is a known issue with the reliability of he sorting event. When the CW and CCW valves are closed the increased pressure in the reactor ring can burst one or several of the Exit channel valves.		#*There is a known issue with the reliability of he sorting event. When the CW and CCW valves are closed the increased pressure in the reactor ring can burst one or several of the Exit channel valves.
-	#**First, close the CW and CCW valves and then check the chip to see if any of the exit valves burst. Then test the automated ~~proecude~~ as decribed below.	+	#**First, close the CW and CCW valves and then check the chip to see if any of the exit valves burst. Then test the automated procedure as decribed below.
	#* be sure the correct chemostat has been selected so the appropriate 'specal valve' will be opened		#* be sure the correct chemostat has been selected so the appropriate 'specal valve' will be opened
-	#*check that no cells escape when all the valves around the sorting chamber are closed. Sometimes if the push-up pressure is too great (or push-~~dwon~~ is too low) the special valve will be forced open and some cells will exit to the feeder channel.	+	#*check that no cells escape when all the valves around the sorting chamber are closed. Sometimes if the push-up pressure is too great (or push-down is too low) the special valve will be forced open and some cells will exit to the feeder channel.

	==Operate the device==		==Operate the device==

Jason R. Kelly: /* Test the device operation */

2006-01-20T16:31:12Z

Test the device operation

←Older revision		Revision as of 16:31, 20 January 2006
Line 49:		Line 49:
	===Test the device operation===		===Test the device operation===
	#Pump		#Pump
-	#*Test if the cells move ~~ine~~	+	#*Test if the cells move well in response to the pump
	#Clean chamber event (on the chamber with the pumps - chamber 8)		#Clean chamber event (on the chamber with the pumps - chamber 8)
-	#* Anecdotally, if the ~~puThis~~ seems to ~~make the pumps work~~, why?	+	#* Anecdotally, if the pumps are not working well this seems to help, why?
-	#** should try just cleaning a random chamber to see if it has same effect, might be a pressure issue, since the channel is higly pressurized following the inputting of cells.	+	#**should try just cleaning a random chamber to see if it has same effect, might be a pressure issue, since the channel is higly pressurized following the inputting of cells.
	#Screening event		#Screening event
	#*confirm that the images in the 2 debug windows show up (both FL and BF). If they don't try restarting the camera. Also in the device section of 'my computer' in windows you can check if the camera is working properly.		#*confirm that the images in the 2 debug windows show up (both FL and BF). If they don't try restarting the camera. Also in the device section of 'my computer' in windows you can check if the camera is working properly.

Jason R. Kelly: /* Test the device operation */

2006-01-20T16:30:27Z

Test the device operation

←Older revision		Revision as of 16:30, 20 January 2006
Line 48:		Line 48:

	===Test the device operation===		===Test the device operation===
-	#~~Run a clean~~ chamber ~~operation~~ on the chamber with the pumps (chamber 8)	+	#Pump
-	#* ~~This~~ seems to make the pumps work, why?	+	#*Test if the cells move ine
		+	#Clean chamber event (on the chamber with the pumps - chamber 8)
		+	#* Anecdotally, if the puThis seems to make the pumps work, why?
	#** should try just cleaning a random chamber to see if it has same effect, might be a pressure issue, since the channel is higly pressurized following the inputting of cells.		#** should try just cleaning a random chamber to see if it has same effect, might be a pressure issue, since the channel is higly pressurized following the inputting of cells.
	#Screening event		#Screening event
	#*confirm that the images in the 2 debug windows show up (both FL and BF). If they don't try restarting the camera. Also in the device section of 'my computer' in windows you can check if the camera is working properly.		#*confirm that the images in the 2 debug windows show up (both FL and BF). If they don't try restarting the camera. Also in the device section of 'my computer' in windows you can check if the camera is working properly.
	#Sorting event		#Sorting event
-	#* There is a known issue with the reliability of he sorting event. When the CW and CCW valves are closed the increased pressure in the reactor ring can burst one or several of the Exit channel valves.	+	#*There is a known issue with the reliability of he sorting event. When the CW and CCW valves are closed the increased pressure in the reactor ring can burst one or several of the Exit channel valves.
	#**First, close the CW and CCW valves and then check the chip to see if any of the exit valves burst. Then test the automated proecude as decribed below.		#**First, close the CW and CCW valves and then check the chip to see if any of the exit valves burst. Then test the automated proecude as decribed below.
	#* be sure the correct chemostat has been selected so the appropriate 'specal valve' will be opened		#* be sure the correct chemostat has been selected so the appropriate 'specal valve' will be opened

Jason R. Kelly: /* Test the device operation */

2006-01-20T16:26:11Z

Test the device operation

←Older revision		Revision as of 16:26, 20 January 2006
Line 51:		Line 51:
	#* This seems to make the pumps work, why?		#* This seems to make the pumps work, why?
	#** should try just cleaning a random chamber to see if it has same effect, might be a pressure issue, since the channel is higly pressurized following the inputting of cells.		#** should try just cleaning a random chamber to see if it has same effect, might be a pressure issue, since the channel is higly pressurized following the inputting of cells.
-	#~~Run~~ a sorting event	+	#Screening event
		+	#*confirm that the images in the 2 debug windows show up (both FL and BF). If they don't try restarting the camera. Also in the device section of 'my computer' in windows you can check if the camera is working properly.
		+	#Sorting event
		+	#* There is a known issue with the reliability of he sorting event. When the CW and CCW valves are closed the increased pressure in the reactor ring can burst one or several of the Exit channel valves.
		+	#**First, close the CW and CCW valves and then check the chip to see if any of the exit valves burst. Then test the automated proecude as decribed below.
	#* be sure the correct chemostat has been selected so the appropriate 'specal valve' will be opened		#* be sure the correct chemostat has been selected so the appropriate 'specal valve' will be opened
	#*check that no cells escape when all the valves around the sorting chamber are closed. Sometimes if the push-up pressure is too great (or push-dwon is too low) the special valve will be forced open and some cells will exit to the feeder channel.		#*check that no cells escape when all the valves around the sorting chamber are closed. Sometimes if the push-up pressure is too great (or push-dwon is too low) the special valve will be forced open and some cells will exit to the feeder channel.
-	~~#Run a screening event~~
-	#*confirm that the images in the 2 debug windows show up (both FL and BF). If they don't try restarting the camera. Also in the device section of 'my computer' in windows you can check if the camera is working properly.

	==Operate the device==		==Operate the device==

Bryanh: /* Operate the device */

2006-01-19T22:35:55Z

Operate the device

←Older revision		Revision as of 22:35, 19 January 2006
Line 63:		Line 63:
	#Test the sort and screen, etc.		#Test the sort and screen, etc.
	#What other tests should be run?		#What other tests should be run?
-		+	*Turn MatLab on first

	===Operating Parameters===		===Operating Parameters===

Bryanh: /* Load the cells into the chemostat */

2006-01-19T19:13:10Z

Load the cells into the chemostat

←Older revision		Revision as of 19:13, 19 January 2006
Line 35:		Line 35:
	#*This helps reduce the likelihood that a large piece of debris clogs one of the channels.		#*This helps reduce the likelihood that a large piece of debris clogs one of the channels.
	#*Use a long tube since it helps to be able to sit at the computer and hold the syringe.		#*Use a long tube since it helps to be able to sit at the computer and hold the syringe.
-	#Close all ~~val~~	+	#Close all valves - once you insert the cells tube be very careful of contaminatoin of the fluid inlets.
	#Remove the center waste tube and insert the cells tube there		#Remove the center waste tube and insert the cells tube there
	#Open channels leading from the center waste channel to the lower waste channel and going all the way around the reactor ring.		#Open channels leading from the center waste channel to the lower waste channel and going all the way around the reactor ring.

Bryanh: /* Load the cells into the chemostat */

2006-01-19T19:10:34Z

Load the cells into the chemostat

←Older revision		Revision as of 19:10, 19 January 2006
Line 35:		Line 35:
	#*This helps reduce the likelihood that a large piece of debris clogs one of the channels.		#*This helps reduce the likelihood that a large piece of debris clogs one of the channels.
	#*Use a long tube since it helps to be able to sit at the computer and hold the syringe.		#*Use a long tube since it helps to be able to sit at the computer and hold the syringe.
		+	#Close all val
	#Remove the center waste tube and insert the cells tube there		#Remove the center waste tube and insert the cells tube there
	#Open channels leading from the center waste channel to the lower waste channel and going all the way around the reactor ring.		#Open channels leading from the center waste channel to the lower waste channel and going all the way around the reactor ring.

Jason R. Kelly: /* Materials */

2005-10-06T19:27:33Z

Materials

←Older revision		Revision as of 19:27, 6 October 2005
Line 3:		Line 3:
	*Lysis Buffer		*Lysis Buffer
	**prepare lysis buffer by adding 10mg/ml [[Lysozyme]] to Pierce B-per Reagent.		**prepare lysis buffer by adding 10mg/ml [[Lysozyme]] to Pierce B-per Reagent.
-	*Media (+ antibiotic if necessary)	+	*Media (+ antibiotic if necessary) (+BSA 2mg/ml)
	''Both lysis buffer and media should be filter sterilized to remove any debris which might clog the channels of the chip.''		''Both lysis buffer and media should be filter sterilized to remove any debris which might clog the channels of the chip.''