Sodium acetate

Purpose

• Sodium acetate can be used as carbon source in media as M9 medium or AB medium.

Procurement

• Where can this material be obtained?

Use

• A usual stock solution of sodium-acetate is 20%
• sodium acetate (CH3COONa) has a MW of 82.03 g/mol (anhydrous) and 136.08 g/mol as trihydrate
• To prepare 20% sodium acetate solution you need 33.2g Na-acetate trihydrate in 100ml H₂O.
• For 500ml 20% Na-acetate add 166g Na-acetate trihydrate to 300ml ddH₂O and fill up to 500 ml.
• Solution can be autoclaved.
• Also used for precipitation of nucleic acids at a final concentration of 0.3M (pH 5.2)