Sodium acetate: Difference between revisions
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(New page: ==Purpose== *Sodium acetate can be used as carbon source in media as M9 medium or AB medium. ==Procurement== *Where can this material be obtained? ==Use== *A usual stock solution...) |
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*sodium acetate (CH3COONa) has a MW of 82.03 g/mol (anhydrous) and 136.08 g/mol as trihydrate | *sodium acetate (CH3COONa) has a MW of 82.03 g/mol (anhydrous) and 136.08 g/mol as trihydrate | ||
*To prepare 20% sodium acetate solution you need 33.2g Na-acetate trihydrate in 100ml H<sub>2</sub>O. | *To prepare 20% sodium acetate solution you need 33.2g Na-acetate trihydrate in 100ml H<sub>2</sub>O. | ||
*For 500ml 20% Na-acetate add ddH<sub>2</sub>O | *For 500ml 20% Na-acetate add 166g Na-acetate trihydrate to 300ml ddH<sub>2</sub>O and fill up to 500 ml. | ||
*Solution can be autoclaved. | *Solution can be autoclaved. | ||
*Also used for precipitation of nucleic acids at a final concentration of 0.3M (pH 5.2) | |||
[[Category:Material]][[Category: | [[Category:Material]][[Category:Acids and bases]] | ||
Latest revision as of 21:06, 24 June 2008
Purpose
Procurement
- Where can this material be obtained?
Use
- A usual stock solution of sodium-acetate is 20%
- sodium acetate (CH3COONa) has a MW of 82.03 g/mol (anhydrous) and 136.08 g/mol as trihydrate
- To prepare 20% sodium acetate solution you need 33.2g Na-acetate trihydrate in 100ml H2O.
- For 500ml 20% Na-acetate add 166g Na-acetate trihydrate to 300ml ddH2O and fill up to 500 ml.
- Solution can be autoclaved.
- Also used for precipitation of nucleic acids at a final concentration of 0.3M (pH 5.2)
