Sodium acetate

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*sodium acetate (CH3COONa) has a MW of 82.03 g/mol (anhydrous) and 136.08 g/mol as trihydrate
*sodium acetate (CH3COONa) has a MW of 82.03 g/mol (anhydrous) and 136.08 g/mol as trihydrate
*To prepare 20% sodium acetate solution you need 33.2g Na-acetate trihydrate in 100ml H<sub>2</sub>O.
*To prepare 20% sodium acetate solution you need 33.2g Na-acetate trihydrate in 100ml H<sub>2</sub>O.
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*For 500ml 20% Na-acetate add ddH<sub>2</sub>O to 166g Na-acetate trihydrate up to 500 ml.
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*For 500ml 20% Na-acetate add 166g Na-acetate trihydrate to 300ml ddH<sub>2</sub>O and fill up to 500 ml.
*Solution can be autoclaved.
*Solution can be autoclaved.
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*Also used for precipitation of nucleic acids at a final concentration of 0.3M (pH 5.2)
[[Category:Material]][[Category:Acids and bases]]
[[Category:Material]][[Category:Acids and bases]]

Current revision

Purpose

Procurement

  • Where can this material be obtained?

Use

  • A usual stock solution of sodium-acetate is 20%
  • sodium acetate (CH3COONa) has a MW of 82.03 g/mol (anhydrous) and 136.08 g/mol as trihydrate
  • To prepare 20% sodium acetate solution you need 33.2g Na-acetate trihydrate in 100ml H2O.
  • For 500ml 20% Na-acetate add 166g Na-acetate trihydrate to 300ml ddH2O and fill up to 500 ml.
  • Solution can be autoclaved.
  • Also used for precipitation of nucleic acids at a final concentration of 0.3M (pH 5.2)
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