Smolke:Protocols/Yeast Media Recipes: Difference between revisions
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'''To use as working media:''' | '''To use as working media:''' | ||
*After autoclaving add: | *After autoclaving add: | ||
**100mL 10X (20%) Raffinose | **100mL 10X (20%) Raffinose (BD 217410) | ||
**100mL 10X (10%) Sucrose | **100mL 10X (10%) Sucrose (Sigma S3929) | ||
**100mL 10X Drop Out stock | **100mL 10X Drop Out stock | ||
**1 L Total Volume | **1 L Total Volume |
Revision as of 22:07, 17 February 2013
Yeast Nitrogen Base Minus Amino Acids (YNB-AA) Recipe
168355)
Autoclave (store at RT for media stocks) To use as working media:
Yeast Nitrogen Base Minus Amino Acids Non-Inducing, Non-Repressing (YNB-AA NINR) Recipe
To use as working media:
Yeast Peptone (YP) Media Recipe
Autoclave (store at RT for media stocks) To use as working media: After autoclaving add:
To pour any of these media stocks as plates:
For yeast KanMX (G418) selection:
-Michael S. Siddiqui 21:53, 4 January 2011 (EST)* 01/04/2011 (based mainly on communications from Andrew Babiskin) |