Smolke:Protocols/Yeast Media Recipes: Difference between revisions
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'''For yeast KanMX (G418) selection:''' | '''For yeast KanMX (G418) selection:''' | ||
* | *Cannot use ammonium sulfate as primary nitrogen source | ||
*Add G418 to YP or to YNB with 1 g/l MSG instead of ammounium sulfate | |||
*Frozen stock is good for 3 months. | *Frozen stock is good for 3 months. | ||
*dry G418 is stored in chemical room. | *dry G418 is stored in chemical room. | ||
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*Add 200mg/L for maintenance (in liquid culture media) | *Add 200mg/L for maintenance (in liquid culture media) | ||
-'''[[User:Michael S. Siddiqui|Michael S. Siddiqui]] 21:53, 4 January 2011 (EST)'''* 01/04/2011 (based mainly on communications from [[User:Andrewb|Andrew Babiskin]]) | -'''[[User:Michael S. Siddiqui|Michael S. Siddiqui]] 21:53, 4 January 2011 (EST)'''* 01/04/2011 (based mainly on communications from [[User:Andrewb|Andrew Babiskin]]) and Dan Kvitek |
Revision as of 13:54, 5 February 2013
Yeast Nitrogen Base Minus Amino Acids (YNB-AA) Recipe
Autoclave (store at RT for media stocks) To use as working media:
Yeast Nitrogen Base Minus Amino Acids Non-Inducing, Non-Repressing (YNB-AA NINR) Recipe
To use as working media:
Yeast Peptone (YP) Media Recipe
Autoclave (store at RT for media stocks) To use as working media: After autoclaving add:
To pour any of these media stocks as plates:
For yeast KanMX (G418) selection:
-Michael S. Siddiqui 21:53, 4 January 2011 (EST)* 01/04/2011 (based mainly on communications from Andrew Babiskin) and Dan Kvitek |