Smolke:Protocols/Western

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(New page: {{Smolke_Top}} ==Overview== Replace this sentence with a brief description of the protocol and its goal. ==Materials== List reagents, supplies and equipment necessary to perform the pro...)
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==Materials==
==Materials==
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List reagents, supplies and equipment necessary to perform the protocol here.  For those materials which have their own OWW pages, link to that page.  Alternatively, links to the suppliers' page on that material are also appropriate.
 
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*supply 1 (i.e. tubes of a certain size? spreaders?)
 
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*reagent 1
 
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*X μL reagent 2
 
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**component A (reagent 2 is made up of multiple components)
 
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**component B
 
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*equipment 1
 
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*equipment 2
 
==Procedure==
==Procedure==
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#Step 1
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#Step 2
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#*Step 2 has some additional information that goes with it.  i.e. Keep at 4°C.
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#Step 3
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##Step 3 has multiple sub-steps within it.
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##Enumerate each of those.
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==Notes==
==Notes==
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Please feel free to post comments, questions, or improvements to this protocol. Happy to have your input!
 
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#List troubleshooting tips here. 
 
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#You can also link to FAQs/tips provided by other sources such as the manufacturer or other websites.
 
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#Anecdotal observations that might be of use to others can also be posted here. 
 
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Please sign your name to your note by adding <font face="courier"><nowiki>'''*~~~~''':</nowiki></font> to the beginning of your tip.
 
==References==
==References==
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'''Relevant papers and books'''
 
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<!-- If this protocol has papers or books associated with it, list those references here.  See the [[OpenWetWare:Biblio]] page for more information. -->
 
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<biblio>
 
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#Goldbeter-PNAS-1981 pmid=6947258
 
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#Jacob-JMB-1961 pmid=13718526
 
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#Ptashne-Genetic-Switch isbn=0879697164
 
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</biblio>
 
==Contact==
==Contact==
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[[Josh Michener]]
[[Category:Protocol]]
[[Category:Protocol]]
[[Category:In vitro]]
[[Category:In vitro]]
[[Category:Yeast]]
[[Category:Yeast]]
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Revision as of 15:50, 23 January 2009

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Contents

Overview

Replace this sentence with a brief description of the protocol and its goal.

Materials

Procedure

Notes

References

Contact

Josh Michener

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