Smolke:Protocols/Sequencing

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Elim's recommended sample format At least 50 ng/ul, preferably >100 ng/ul for plasmid sample 3 uM for primers 10 ul plasmid and 3 uL primer for each sequencing reaction Submit 10 uL or 3 uL/rxn of primer, whichever is larger. -Josh Use 8-PCR tube strips I prefer 0.5mL tubes, which give more room to label on the top. -Josh Elim biopharm: When possible, please use 8-strip PCR tubes for less than 96 samples and primers, and 96-well plates for equal to or greater than 96 samples Tape sample to order form Or recycle the bags that primers come in, and just put the order form in the bag. -Josh Notes If sequencing constructs with strong secondary structures, check GC rich on order form. I have found that "GC Protocol 2" often worked better for the various shRNA and miRNA sequences I have submitted, and you could specify this in the comments section on the order form. --YC