Smolke:Protocols/Plasmid prep: Difference between revisions
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*Decant your cultures into 1.5mL Eppendorf tubes to pellet the cells. This saves the cost of a 15mL Falcon tube and/or a 5mL pipet. -Josh | *Decant your cultures into 1.5mL Eppendorf tubes to pellet the cells. This saves the cost of a 15mL Falcon tube and/or a 5mL pipet. -Josh | ||
*Follow Qiagen's QiaPrep Miniprep protocol, but skip step 7 (the Buffer PB wash). | *Follow Qiagen's QiaPrep Miniprep protocol, but skip step 7 (the Buffer PB wash). | ||
*Aliquot buffers from | *Aliquot buffers from working stocks prepared by Andy (stored on bottom shelf of chemical cabinet). | ||
**When you aliquot from the stocks please use careful, sterile technique (even if you don't use sterile technique on your own stocks). | **When you aliquot from the stocks please use careful, sterile technique (even if you don't use sterile technique on your own stocks). | ||
**Take aliquots of ~15 ml of P1, P2, N3 in Falcon tubes and keep on your bench. | **Take aliquots of ~15 ml of P1, P2, N3 in Falcon tubes and keep on your bench. |
Latest revision as of 10:48, 20 July 2010
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