Smolke:Protocols/Pfu Buffer

Summary

10x buffer for PCR with Pfu. Makes 10mL.

Ingredients

• 1M Tris-HCl pH 8.8
• 333 μL 3M KCl
• 500 μL 20% Triton X-100
• 1 mL 100x BSA
• 200 μL 1M MgSO₄
• 5.967 mL H₂O

Protocol

1. Mix, vortex.
2. Store at -20°C

Notes

• We couldn't find any Triton X-100 in the lab. Our best guess is that NP-40 was (previously) used instead. -Jkm 18:18, 2 August 2006 (EDT)

Source

Unknown. Ask Kristy.

Contact

Kristy