Smolke:Protocols/Pfu Buffer: Difference between revisions
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==Summary== | ==Summary== | ||
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==Ingredients== | ==Ingredients== | ||
*1M Tris-HCl pH 8.8 | *2 mL 1M Tris-HCl pH 8.8 | ||
*333 μL 3M KCl | *333 μL 3M KCl | ||
*500 μL 20% Triton X-100 | *500 μL 20% Triton X-100 | ||
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==Notes== | ==Notes== | ||
*We couldn't find any Triton X-100 in the lab. Our best guess is that NP-40 was (previously) used instead. -[[User:Jkm|Jkm]] 18:18, 2 August 2006 (EDT) | *We couldn't find any Triton X-100 in the lab. Our best guess is that NP-40 was (previously) used instead. -[[User:Jkm|Jkm]] 18:18, 2 August 2006 (EDT) | ||
*Should be supplemented with MgCl<sub>2</sub> when used. A working concentration of 2.5mM (a 1/20 dilution of 50mM stock) works for most applications. | |||
==Source== | ==Source== |
Latest revision as of 14:08, 20 October 2009
Summary10x buffer for PCR with Pfu. Makes 10mL. Ingredients
Protocol
Notes
SourceUnknown. Ask Kristy. Contact |