Smolke:Protocols/Insert prep: Difference between revisions
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**250 ng plasmid | **250 ng plasmid | ||
**10 ul 1:10 Taq DNA polymerase | **10 ul 1:10 Taq DNA polymerase | ||
**(Starting Feb/28th, 2013, we will use 5 ul of 1:20 Taq DNA polymerase) | |||
*After PCR, combine products from the five tubes, and run 5 ul on a gel to verify product length | *After PCR, combine products from the five tubes, and run 5 ul on a gel to verify product length | ||
*DpnI treat the PCR products to remove the template - add DpnI directly to the PCR reaction (0.5uL in a 50uL reaction is plenty). Incubate 1hr at 37C. -Josh | *DpnI treat the PCR products to remove the template - add DpnI directly to the PCR reaction (0.5uL in a 50uL reaction is plenty). Incubate 1hr at 37C. -Josh |
Revision as of 23:10, 28 February 2013
Protocol
Notes
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