Smolke:Protocols/General Sterility: Difference between revisions
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* Keep all containers (such as tip boxes, media, cell culture flasks/dishes, etc.) closed when not actively in use. | * Keep all containers (such as tip boxes, media, cell culture flasks/dishes, etc.) closed when not actively in use. | ||
* Do not put dirty pipette tips (or other dirty items) on the work surface or in the tip box. | * Do not put dirty pipette tips (or other dirty items) on the work surface or in the tip box. | ||
* The aspirator flask must be emptied promptly when | * The aspirator flask must be emptied promptly when the liquid level becomes too high. Refill by adding solution of fresh iodine enough to cover the bottom of the aspirator flask. | ||
* In order to minimize contamination and cross contamination, ethanol should be run through the aspirator tip when going between samples. | * In order to minimize contamination and cross contamination, ethanol should be run through the aspirator tip when going between samples. | ||
* In addition, it is best to change glass Pasteur pipette when changing between cell lines and before use. | * In addition, it is best to change glass Pasteur pipette when changing between cell lines and before use. | ||
* Do not abandon your cultures in the incubator. They should be removed and disposed of once they are no longer needed or of use. | * Do not abandon your cultures in the incubator. They should be removed and disposed of once they are no longer needed or of use. |
Latest revision as of 17:46, 11 July 2012
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